Genistein supplementation to the quail: Effects on egg production and egg yolk genistein, daidzein, and lipid peroxidation levels
Genistein supplementation to the quail: Effects on egg production and egg yolk genistein, daidzein, and lipid peroxidation levels
复制标题
DOI：
10.3382/ps.2009-00004
复制
发表时间：
2009-10-01
期刊：
影响因子：
4.4
通讯作者：
Sahin, K.
中科院分区：
文献类型：
--
作者：
Akdemir, F.;Sahin, K.
染料木黄酮，一种大豆植物雌激素，是一种强大的抗氧化剂。本试验旨在研究饲粮中添加染料木黄酮对日本鹌鹑产蛋性能和蛋黄中丙二醛（MDA）、维生素A和维生素E含量的影响。丙二醛是脂质过氧化的指标，而维生素A和E具有抗氧化作用。试验选取5周龄的150只试验禽，随机分为3组，每组50只（5个重复，每10个重复），分别饲喂基础饲粮或在基础饲粮中添加400或800 mg染料木素/kg饲粮。试验期90 d，拍摄时间为17L:7D。作为抗氧化指标，用高效液相色谱法测定蛋黄丙二醛（MDA）和维生素（A、E）浓度。饲粮中添加染料木素（800 mg/kg）显著提高了采食量、产蛋量、蛋重、哈氏单位、壳厚和壳重，并显著提高了饲料效率。在染料木素添加水平最高时，蛋黄染料木素浓度升高（P < 0.0001），蛋黄丙二醛浓度降低（P < 0.0001）。然而，补充染料木素不会影响蛋黄中的大豆苷元、维生素A和维生素E水平。蛋黄染料木素与MDA浓度呈负相关（y = 0.02 x e（染料木素），R-2 = 0.74, P < 0.0001）。结果表明，饲粮中添加800 mg/kg染料木素可提高鹌鹑生产性能和蛋品质，提高蛋黄染料木素含量，降低蛋黄丙二醛浓度。
Genistein, a soy phytoestrogen, is a powerful antioxidant. In the present study, we investigated the effects of dietary genistein supplementation on Japanese quail (Coturnix coturnix japonica) laying performance and egg yolk contents of malondialdehyde (MDA), vitamin A, and vitamin E. Malondialdehyde is an indicator of lipid peroxidation, whereas vitamins A and E have antioxidant properties. Birds (n = 150; 5 wk old) were randomly assigned to 1 of 3 groups consisting of 50 birds (5 replicates of 10) and were fed a basal diet or the basal diet supplemented with either 400 or 800 mg of genistein/kg of diet. The experimental period lasted 90 d with a 17L:7D photo schedule. As antioxidant indices, yolk MDA and vitamin (A and E) concentrations were measured by HPLC. Dietary genistein supplementation (800 mg/kg) increased feed intake, egg production, egg weight, Haugh unit, shell thickness, and shell weight and improved feed efficiency at a greater extent than the other levels (0 and 400 mg/kg). Egg yolk genistein concentration was increased (P < 0.0001), whereas egg yolk MDA concentration was decreased (P < 0.0001) at the highest level of genistein supplementation. However, genistein supplementation did not affect egg yolk daidzein, vitamin A, and vitamin E levels. There was an inverse relationship between egg yolk genistein and MDA concentration (y = 0.02 x e(genistein), R-2 = 0.74, P < 0.0001). Results of the present study indicate that supplementing with dietary genistein (800 mg/kg) improved performance, egg quality, and egg yolk genistein content and decreased egg yolk MDA concentration in quail.