Indicator-based and indicator-free magnetic assays connected with disposable electrochemical nucleic acid sensor system
Indicator-based and indicator-free magnetic assays connected with disposable electrochemical nucleic acid sensor system
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DOI：
10.1016/j.talanta.2008.10.057
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发表时间：
2009-04-15
期刊：
影响因子：
6.1
通讯作者：
Jelen, Frantisek
中科院分区：
文献类型：
--
作者：
Karadeniz, Hakan;Erdem, Arzum;Jelen, Frantisek
成功地建立了基于指示剂和无指示剂的一次性铅笔石墨电极（PGE）磁检测方法，并对其用于DNA杂交的电化学检测进行了比较。采用差分脉冲伏安法（DPV）检测DNA杂交条件下，棘霉素（echinomycin， ECHI）和电活性DNA碱基鸟嘌呤（guanine）和腺嘌呤（adenine）的氧化信号。将生物素化探针固定在磁珠（磁颗粒、微球）上，并利用电化学传感器系统成功地在介质中与颗粒表面的互补靶杂交。对于选择性研究，结果表明，与具有一个碱基或多个错配的双工相比，基于指示剂和无指示剂的磁分析对DNA杂交提供了更好的辨别。利用一次性传感器技术，在靶物的nM浓度水平上发现了基于指示剂或无指示剂的磁法的检出限（S/N = 3），重现性好。还讨论了与一次性电化学传感器连接的两种提出的磁检测的表征和优点，并与文献中先前报道的那些方法进行了比较。(c) 2008 Elsevier B.V.版权所有
An indicator-based and indicator-free magnetic assays connected with a disposable pencil graphite electrode (PGE) were successfully developed, and also compared for the electrochemical detection of DNA hybridization. The oxidation signals of echinomycin (ECHI) and electroactive DNA bases, guanine and adenine, respectively were monitored in the presence of DNA hybridization by using differential pulse voltammetry (DPV) technique. The biotinylated probe was immobilized onto the magnetic beads (magnetic particles, microspheres) and hybridization with its complementary target at the surface of particles within the medium was exhibited successfully using electrochemical sensor system. For the selectivity studies, the results represent that both indicator-based and indicator-free magnetic assays provide a better discrimination for DNA hybridization compared to duplex with one-base or more mismatches. The detection limits (S/N = 3) of the magnetic assays based on indicator or indicator-free were found in nM concentration level of target using disposable sensor technology with good reproducibility. The characterization and advantages of both proposed magnetic assays connected with a disposable electrochemical sensor are also discussed and compared with those methods previously reported in the literature. (c) 2008 Elsevier B.V. All rights reserved.