Adhesion to fibronectin maintains regenerative capacity during ex vivo culture and transduction of human hematopoietic stem and progenitor cells
Adhesion to fibronectin maintains regenerative capacity during ex vivo culture and transduction of human hematopoietic stem and progenitor cells
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DOI：
10.1182/blood.v92.12.4612.424k04_4612_4621
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发表时间：
1998-12-15
期刊：
影响因子：
20.3
通讯作者：
Nolta, JA
中科院分区：
文献类型：
--
作者：
Dao, MA;Hashino, K;Nolta, JA
近年来的研究表明，造血干细胞（HSC）在体外的移植能力很差，然而，在体外干细胞扩增和逆转录病毒介导的基因治疗领域，诱导细胞在培养中循环是至关重要的。通过使用异种移植模型，目前的数据表明，只有当人类造血干细胞和祖细胞有机会将其整合素受体与纤连蛋白结合时，它们才能在体外穿越M期、整合逆转录病毒载体、移植并维持长期造血。在培养期间。如果在相同条件下悬浮培养，则转导是不可检测的，并且原始细胞的长期多谱系再生能力严重减弱。(C)1998年，美国血液学会。
Recent reports have indicated that there is poor engraftment from hematopoietic stem cells (HSC) that have traversed cell cycle ex vivo, However, inducing cells to cycle in culture is critical to the fields of ex vivo stem cell expansion and retroviral-mediated gene therapy. Through the use of a xenograft model, the current data shows that human hematopoietic stem and progenitor cells can traverse M phase ex vivo, integrate retroviral vectors, engraft, and sustain longterm hematopoiesis only if they have had the opportunity to engage their integrin receptors to fibronectin during the culture period. If cultured in suspension under the same conditions, transduction is undetectable and the long-term multilineage regenerative capacity of the primitive cells is severely diminished. (C) 1998 by The American Society of Hematology.