Influenza A virus PB1-F2 gene in recent Taiwanese isolates
Influenza A virus PB1-F2 gene in recent Taiwanese isolates
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DOI：
10.3201/eid1004.030412
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发表时间：
2004-04-01
影响因子：
11.8
通讯作者：
Shih, SR
中科院分区：
文献类型：
--
作者：
Chen, GW;Yang, CC;Shih, SR
甲型流感病毒含有 8 个 RNA 片段，编码 10 种病毒蛋白。然而，在 A/Puerto Rico/8/34 (H1N1) 中发现了第 11 种蛋白质，称为 PB1-F2。这种新型蛋白质是从 PB1 基因中的另一个开放阅读框 (ORF) 翻译而来。我们分析了台湾近期分离的 42 株甲型流感病毒株的 PB1 基因，其中包括 24 株 H1N1 株和 18 株 H3N2 株。 1 个 H1N1 分离株和 17 个 H3N2 分离株包含 90 个残基的完整 PB1-F2 ORF，比 C 端 A/Puerto Rico/8/34 的 PB1-F2 长三个氨基酸 (aa)。剩下的一个 H3N2 分离株编码具有 79 个残基的截短 PB1-F2。其他 23 个 H1N1 分离株含有 57 个氨基酸的截短 PB1-F2。 HA 和 PB1 基因的系统发育分析表明，这些台湾分离株的聚类相似，表明这两个基因组片段之间没有发生明显的重配。
Influenza A virus contains eight RNA segments and encodes 10 viral proteins. However, an 11th protein, called PB1-F2, was found in A/Puerto Rico/8/34 (H1N1). This novel protein is translated from an alternative open reading frame (ORF) in the PB1 gene. We analyzed the PB1 gene of 42 recent influenza A isolates in Taiwan, including 24 H1N1 and 18 H3N2 strains. One H1N1 isolate and 17 H3N2 isolates contained the entire PB1-F2 ORF of 90 residues, three amino acids (aa) longer than the PB1-F2 of A/Puerto Rico/8/34 at the C terminal. The one remaining H3N2 isolate encoded a truncated PB1-F2 with 79 residues. The other 23 H1N1 isolates contained a truncated PB1-F2 of 57 aa. Phylogenetic analysis of both the HA and the PB1 genes showed that they shared similar clustering of these Taiwanese isolates, suggesting that no obvious reassortment occurred between the two genomic segments.