Monitoring of CD34+ cells during leukapheresis allows a single, successful collection of hemopoietic progenitors in patients with low numbers of circulating stem cells
Monitoring of CD34+ cells during leukapheresis allows a single, successful collection of hemopoietic progenitors in patients with low numbers of circulating stem cells
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在白细胞分离过程中监测 CD34+ 细胞可以在循环干细胞数量较少的患者中成功收集造血祖细胞
DOI：
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发表时间：
1999
影响因子：
4.8
通讯作者：
Franco Mandelli
中科院分区：
文献类型：
--
作者：
P. Fabritiis;Marcos González;Giovanna Meloni;M. S. D. Propris;Roberto Bellucci;Iole Cordone;M. Gozzer;G. Leone;Franco Mandelli
我们研究了188例接受G-CSF动员治疗或不接受化疗的恶性血液病患者，目的是：(1)建立循环祖细胞的下限，允许单次白细胞分离收集2 × 106CD34+细胞/kg，使用标准参数设置的仪器；(2)评价采集到的CD34+细胞数量和质量在白细胞分离过程中是否保持稳定；(3)在使用标准参数设置的仪器无法达到目标的患者中，通过单一程序收集足够数量的循环CD34+细胞。对85例患者进行的回顾性分析表明，19个循环CD34+细胞/μl代表能够区分需要一次或多次单采收集2 × 106个CD34+细胞/kg的患者的截止数字。在随后的70例患者中前瞻性地证实了该值的有效性。体外实验结果显示，在白细胞分离过程中，患者血液中CD34+细胞的数量、不同的CD34+细胞亚群的比例和干细胞室的克隆生成能力都是稳定的，根据这些结果，我们调整了33例患者的血容量，CD34+细胞/μl <19 pb。在白细胞分离过程中监测干细胞的收集，并延长该过程的时间，估计足以通过单一过程达到CD34+细胞的目标数量。由仪器自动设定的血量计算得到的处理总血量增量中位数为25.2%，处理总血量增量中位数为3.3倍。在所有病例中，充分的CD34+细胞收集在一个单一的程序中完成。自体移植后，血液重建模式与其他所有患者相似。
We have studied a total of 188 patients with hematological malignancies, submitted to mobilization therapy with G-CSF associated or not with chemotherapy in order to: (1) establish the lower limit of circulating progenitor cells that allows the collection of 2 × 106CD34+ cells/kg by a single leukapheresis, utilizing the instrument set on standard parameters; (2) evaluate whether the number and quality of CD34+ cells collected remain stable during leukapheresis; and (3) collect a sufficient number of circulating CD34+ cells by a single procedure in patients in whom such an approach would have been insufficient to reach the target with the instrument set on standard parameters. The retrospective analysis conducted in 85 patients showed that 19 circulating CD34+ cells/μl represented the cut-off number capable of discriminating between patients who will require one or more apheresis to collect 2 × 106 CD34+ cells/kg. The validity of this value was prospectively confirmed in 70 subsequent patients. Based on in vitroresults that showed the stability in the number of CD34+ cells, the proportion of different CD34+ cell subpopulations and the clonogenic capacity of the stem cell compartment during leukapheresis both in the blood of the patients and in samples taken directly from the instrument, we have adapted the blood volume to be processed in 33 patients with <19 pb cd34+ cells/μl. Stem cell collection was monitored during the leukapheresis and the procedure was prolonged for a time period estimated to be sufficient to reach the target number of CD34+ cells with a single procedure. The median increment of the total blood volume processed, calculated from the volume set automatically by the instrument was 25.2%, with a median of 3.3-fold total blood volume processed. In all cases, a sufficient CD34+ cell collection was completed in a single procedure. After autograft, the pattern of blood reconstitution was similar to that of all the other patients.
影响因子：
158.5
作者：
SANTOS, GW;TUTSCHKA, PJ;YEAGER, AM
通讯作者：
YEAGER, AM
影响因子：
20.3
作者：
Felipe Prósper;Kirk Vanoverbeke;David F. Stroncek;Catherine M. Verfaillie
通讯作者：
Felipe Prósper;Kirk Vanoverbeke;David F. Stroncek;Catherine M. Verfaillie