Measurements of the molecular weight variability of plasma low density lipoproteins among normals and subjects with hyper- -lipoproteinemia. Demonstration of macromolecular heterogeneity.
Measurements of the molecular weight variability of plasma low density lipoproteins among normals and subjects with hyper- -lipoproteinemia. Demonstration of macromolecular heterogeneity.
复制标题
正常人和高脂蛋白血症受试者血浆低密度脂蛋白分子量变异性的测量。
DOI：
10.1021/bi00754a006
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发表时间：
1972
期刊：
影响因子：
2.9
通讯作者：
G. Warmke
中科院分区：
文献类型：
--
作者：
W. Fisher;M. Hammond;G. Warmke
方法通过加入固体KBr将血清密度调节至1.20 g/cm 3后，通过超离心从血清中分离总脂蛋白级分（Del Gatto et al.，1959），而LDL级分的分离是通过如前所述的在制备型超离心器中的差密度浮选进行的（De Lalla和Gofman，1954; Maul-din和Fisher，1970）。在这些研究中，血清最初是生物化学，第11卷，第4期，1972 519
MethodsThe total lipoprotein fraction was isolated from serum by ultracentrifugation after adjusting the density of the serum to 1.20 g/cm3by the addition of solid KBr (Del Gatto et al., 1959), while isolation of the LDL fraction was performed by differential density flotation in the preparative ultracentrifuge as previously described (De Lalla and Gofman, 1954; Maul-din and Fisher, 1970). In these studiesthe serum was initially biochemistry, vol. 11, no. 4, 1972 519