Construction of Pedicled Smooth Muscle Tissues by Combining the Capsule Tissue and Cell Sheet Engineering
Construction of Pedicled Smooth Muscle Tissues by Combining the Capsule Tissue and Cell Sheet Engineering
复制标题
荚膜组织与细胞片工程相结合构建带蒂平滑肌组织
DOI：
10.1177/0963689718821682
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发表时间：
2019-03-01
影响因子：
3.3
通讯作者：
Chen, Fang
中科院分区：
文献类型：
--
作者：
Jia, Zhiming;Guo, Hailin;Chen, Fang
工程组织的存活需要其自身毛细血管网络的形成，并在移植后与宿主血管相吻合。目前，虽然研究了许多策略，如修饰支架材料，添加内皮细胞或血管生成因子，但工程组织植入体内不能及时获得足够的血液供应，导致缺血细胞凋亡或收缩。构建具有自身轴向血管的工程组织并进行带蒂移植，有望解决组织工程中血管化问题。在这项研究中，我们使用组织扩张囊作为一种新的平台，用于血管化自体平滑肌细胞（SMC）薄片，并制造具有自身血管蒂的血管化工程组织。首先，我们验证了哪个时间点对构建轴向囊血管床最有效。其次，我们比较了SMC片移植到扩张囊和经典背侧皮下组织的结果，后者在其他研究中广泛用于血管化。最后，我们在囊床上移植了两次多层SMC薄片，验证了制造带蒂厚工程平滑肌组织的可行性。结果表明，轴向囊组织可成功诱导，充分膨胀后1周的囊组织血管化程度最高。通过对两种血管床上细胞片的厚度、血管密度和凋亡的定量比较，证明轴向包膜血管床比传统皮下组织更有利于移植物的生长和血管化。此外，通过在囊床上多次移植细胞片，可以构建具有血管蒂的厚血管化平滑肌组织。轴向包膜维管床与细胞片工程相结合，为克服组织工程中血管形成缓慢或不足的问题提供了一种有效的策略。
The survival of engineered tissue requires the formation of its own capillary network, which can anastomose with the host vasculature after transplantation. Currently, while many strategies, such as modifying the scaffold material, adding endothelial cells, or angiogenic factors, have been researched, engineered tissue implanted in vivo cannot timely access to sufficient blood supply, leading to ischemic apoptosis or shrinkage. Constructing vascularized engineered tissue with its own axial vessels and subsequent pedicled transfer is promising to solve the problem of vascularization in tissue engineering. In this study, we used the tissue expander capsule as a novel platform for vascularizing autologous smooth muscle cell (SMC) sheets and fabricating vascularized engineered tissue with its own vascular pedicle. First, we verified which time point was the most effective for constructing an axial capsule vascular bed. Second, we compared the outcome of SMC sheet transplantation onto the expander capsule and classical dorsal subcutaneous tissue, which was widely used in other studies for vascularization. Finally, we transplanted multilayered SMC sheets onto the capsule bed twice to verify the feasibility of fabricating thick pedicled engineered smooth muscle tissues. The results indicated that the axial capsule tissue could be successfully induced, and the capsule tissue 1 week after full expansion was the most vascularized. Quantitative comparisons of thickness, vessel density, and apoptosis of cell sheet grafts onto two vascular beds proved that the axial capsule vascular bed was more favorable to the growth and vascularization of transplants than classical subcutaneous tissue. Furthermore, thick vascularized smooth muscle tissues with the vascular pedicle could be constructed by multi-transplanting cell sheets onto the capsule bed. The combination of axial capsule vascular bed and cell sheet engineering may provide an efficient strategy to overcome the problem of slow or insufficient vascularization in tissue engineering.