Analysis of the methylation status of genes up-regulated by the demethylating agent, 5-aza-2′-deoxycytidine, in esophageal squamous cell carcinoma
Analysis of the methylation status of genes up-regulated by the demethylating agent, 5-aza-2′-deoxycytidine, in esophageal squamous cell carcinoma
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DOI：
10.3892/or_00000022
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发表时间：
2008-08-01
期刊：
影响因子：
4.2
通讯作者：
Yokosuka, Osamu
中科院分区：
文献类型：
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作者：
Arai, Makoto;Imazeki, Fumio;Yokosuka, Osamu
为了确定基因启动子甲基化在食管鳞状细胞癌（ESCC）中的临床意义，我们通过基于DNA微阵列的转录组学分析，检测了3种ESCC细胞系（TE-1、TE-2、TE-10）在5-aza-2'-脱氧胞苷（DAC）治疗后基因启动子甲基化表达升高的状态。我们观察到在组织蛋白酶L2 （CTSL2）、食管正常粘膜特异性1 （NMES1）和脂肪酸结合蛋白5 （FABP5）这三个基因的启动子区域存在高度的DNA甲基化。ESCC细胞系中NMES1的过表达增加了细胞的运动性。NMES1的下调可能在ESCC的细胞运动中起重要作用，或者是恶性肿瘤的一个有效标志。
To determine the clinical significance of gene promoter methylation in esophageal squamous cell carcinoma (ESCC), we examined the promoter methylation status of genes showing elevated expression, as determined by DNA microarray-based transcriptomic analysis, in three ESCC cell lines (TE-1, TE-2, TE-10) after 5-aza-2'-deoxycytidine (DAC) treatment. We observed a high degree of DNA methylation within the promoter regions of three genes, namely cathepsin L2 (CTSL2), normal mucosa of esophagus specific 1 (NMES1), and fatty acid binding protein 5 (FABP5). Overexpression of NMES1 in ESCC cell lines increased cell motility. Downregulation of NMES1 might play an important role in the cell motility of ESCC or be a potent marker of malignancy.