In situ hybridization analysis of gibbon chromosomes suggests that amplification of alpha satellite DNA in the telomere region is confined to two of the four genera
In situ hybridization analysis of gibbon chromosomes suggests that amplification of alpha satellite DNA in the telomere region is confined to two of the four genera
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DOI：
10.1139/gen-2012-0123
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发表时间：
2012-11-01
期刊：
影响因子：
3.1
通讯作者：
Hirai, Hirohisa
中科院分区：
文献类型：
--
作者：
Baicharoen, Sudarath;Arsaithamkul, Visit;Hirai, Hirohisa
siamang （Symphalangus syndactylus）是长臂猿科（长臂猿）的一种，在染色体的端粒区携带大量的组成异染色质。我们最近发现，α卫星DNA是构成这些异染色质块的主要成分。α卫星DNA是灵长类动物着丝粒的主要组成部分，是171 bp重复单元的串联重复序列。除了暹罗猴，白颊长臂猿（Nomascus leucogenys）之前也被发现在端粒区域携带α卫星DNA，尽管没有暹罗猴那么大。长臂猿包括4个属：长尾猿、舌尾猿、无尾猿和舌尾猿。在这里，我们报告了α卫星DNA在端粒区域的扩增可能仅限于两个属：Nomascus和Symphalangus。我们研究了一种Hoolock和四种hyloates，并获得了在这些物种中反对这种扩增事件的证据。长臂猿四属的系统发育关系尚不清楚。对于目前端粒区α卫星DNA的分布，一个简单的解释是Nomascus和Symphalangus的亲缘关系相对较近，扩增发生在它们共同的祖先身上。
The siamang (Symphalangus syndactylus), a species of the family Hylobatidae (gibbons), carries large blocks of constitutive heterochromatin in the telomere region of chromosomes. We recently found that alpha satellite DNA constitutes these heterochromatin blocks as a main component. Alpha satellite DNA, tandem repeat sequences of 171-bp repeat units, is a major component of centromeres in primates. In addition to the siamang, the white-cheeked gibbon (Nomascus leucogenys) was previously found to carry the alpha satellite DNA in the telomere region, although not as large a scale as the siamang. Gibbons comprise four genera: Hoolock, Hylobates, Nomascus, and Symphalangus. Here, we report that the amplification of alpha satellite DNA in the telomere region is probably confined to two genera: Nomascus and Symphalangus. We examined one species of Hoolock and four species of Hylobates and obtained evidence against such an amplification event in these species. The phylogenetic relationship of the four gibbon genera remains unclear. One simple explanation for the current distribution of the telomere region alpha satellite DNA would be that Nomascus and Symphalangus are relatively closely related and the amplification occurred in their common ancestor.