Heme oxygenase-1 expression in chronic rhinosinusitis with eosinophilic infiltration
Heme oxygenase-1 expression in chronic rhinosinusitis with eosinophilic infiltration
复制标题
DOI：
10.1016/j.anl.2011.10.001
复制
发表时间：
2012-08-01
影响因子：
1.7
通讯作者：
Ikeda, Katsuhisa
中科院分区：
文献类型：
--
作者：
Kawano, Kenji;Kusunoki, Takeshi;Ikeda, Katsuhisa
目的：慢性鼻窦炎伴嗜酸性粒细胞浸润是一种难治性鼻窦炎，常伴发哮喘。众所周知，氧化剂会导致哮喘加重。血红素氧合酶-1（HO-1）是一种抗氧化的细胞保护酶，在气道疾病中已被广泛研究。方法：取CRS鼻息肉组织标本，分别取嗜酸性粒细胞浸润和非嗜酸性粒细胞浸润的标本，分别行HE染色和免疫组化染色，检测HO-1和巨噬细胞的表达。我们发现，与有嗜酸性粒细胞浸润的CRS相比，无嗜酸性粒细胞浸润的CRS上皮层中HO-1的表达显著增强。另一方面，与HO-1阳性反应的巨噬细胞的数量显着增加，在CRS与嗜酸性粒细胞浸润相比，CRS没有eosinophilic infiltration.Conclusions：我们的研究表明，无论是减少HO-1在上皮细胞的表达和HO-1阳性的巨噬细胞浸润的增加与CRS与嗜酸性粒细胞浸润的上皮损伤。© 2011 Elsevier爱尔兰有限公司版权所有。
Objectives: Chronic rhinosinusitis (CRS) with eosinophilic infiltration is a type of intractable rhinosinusitis often associated with asthma. The oxidants are well known to induce aggravate asthma. Heme oxygenase-1 (HO-1), a cytoprotective enzyme against oxidant, has been extensively studied in airway diseases. However, no study that observed HO-1 in both epithelial and subepithelial tissues of CRS has been reported.Methods: Part of each specimen derived from the nasal polyps of CRS with and without eosinophilic infiltration was promptly fixed for hematoxylin-eosin staining and immunohistochemical analysis for HO-1 and macrophages.Results: We found that the expression of HO-1 in the epithelial layers of CRS without eosinophilic infiltration was significantly enhanced as compared with that of CRS with eosinophilic infiltration. On the other hand, the number of macrophages with HO-1 positive reactions was significantly greater in CRS with eosinophilic infiltration compared with CRS without eosinophilic infiltration.Conclusions: Our study suggests that both a reduction of HO-1 expression in epithelial cells and an increase of infiltration of macrophages positive for HO-1 are related to the epithelial damage of CRS with eosinophilic infiltration. to (C) 2011 Elsevier Ireland Ltd. All rights reserved.