Functional cardiac cell constructs on cellulose-based scaffolding
Functional cardiac cell constructs on cellulose-based scaffolding
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DOI：
10.1016/j.biomaterials.2004.01.024
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发表时间：
2004-11-01
期刊：
影响因子：
14
通讯作者：
Kostov, Y
中科院分区：
文献类型：
--
作者：
Entcheva, E;Bien, H;Kostov, Y
纤维素及其衍生物已成功地用作各种应用的生物材料，包括透析膜、生物传感器中的扩散限制膜。在体外中空纤维灌注系统，表面的细胞扩增等，在这项研究中，我们测试了醋酸纤维素（CA）和再生纤维素（RC）的支架在培养中生长功能性心脏细胞结构的潜力。具体来说，我们证明CA和RC表面促进心脏细胞生长，增强细胞连接（间隙连接）和电功能。作为光学透明和基本上非自发荧光，CA支架不干扰细胞构建体中的功能光学测量。按照精细的细节或复杂的三维形状成型是组织工程中支架设计的额外重要特征。生物降解性可通过CA的水解、脱乙酰化和细胞相容性酶（纤维素酶）作用来控制，最终产物为葡萄糖。在体外培养心脏细胞和生长组织样心脏构建体可以有益于纤维素支架的多功能性和可接近性，结合良好的粘附性（与标准组织培养物处理的聚苯乙烯相当）、低至纳米级的模塑能力（与目前在软光刻中最受欢迎的聚二甲基硅氧烷相当）和受控的生物降解性。(C)2004 Elsevier Ltd.保留所有权利。
Cellulose and its derivatives have been successfully employed as biomaterials in various applications, including dialysis membranes, diffusion-limiting membranes in biosensors. in vitro hollow fibers perfusion systems, surfaces for cell expansion, etc. In this study, we tested the potential of cellulose acetate (CA) and regenerated cellulose (RC) scaffolds for growing functional cardiac cell constructs in culture. Specifically, we demonstrate that CA and RC surfaces are promoting cardiac cell growth, enhancing cell connectivity (gap junctions) and electrical functionality. Being optically clear and essentially non-autofluorescent, CA scaffolds did not interfere with functional optical measurements in the cell constructs. Molding to follow fine details or complex three-dimensional shapes are additional important characteristics for scaffold design in tissue engineering. Biodegradability can be controlled by hydrolysis, de-acetylization of CA and cytocompatible enzyme (cellulase) action, with glucose as a final product. Culturing of cardiac cells and growth of tissue-like cardiac Constructs in vitro could benefit front the versatility and accessibility of cellulose scaffolds, combining good adhesion (comparable to the standard tissue-culture treated polystyrene), molding capabilities down to the nanoscale (comparable to the current favorite in soft lithograpliy-polydimethylsiloxane) with controlled biodegradability. (C) 2004 Elsevier Ltd. All rights reserved.