The effect of settling on cytotoxicity evaluation of SiO2 nanoparticles
The effect of settling on cytotoxicity evaluation of SiO2 nanoparticles
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DOI：
10.1016/j.jaerosci.2017.02.011
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发表时间：
2017-06-01
影响因子：
4.5
通讯作者：
Pratsinis, Sotiris E.
中科院分区：
文献类型：
--
作者：
Spyrogianni, Anastasia;Sotiriou, Georgios A.;Pratsinis, Sotiris E.
纳米材料的体外细胞毒性通常通过监测暴露于其悬浮液下的细胞活力来评估。对于相同的标称纳米颗粒浓度，递送至细胞的实际纳米颗粒剂量可能取决于它们的沉降速率。这里的目标是定量评估沉降对于气溶胶制造的分形 SiO2 纳米颗粒体外细胞毒性评估的重要性，这些纳米颗粒具有相似的团聚体尺寸和结构，但初级粒径不同。它们的沉积率通过紫外可见光谱测量，并在标准直立和倒置鼠巨噬细胞培养配置中评估其体外细胞毒性。在标准直立细胞培养物中，较大初级颗粒的二氧化硅比较小初级颗粒的二氧化硅沉降得更快，并且显得更具细胞毒性。相比之下，在倒置细胞培养物中，所有二氧化硅都表现出相似且低得多的细胞毒性，揭示了纳米颗粒沉降在以 H2O2 溶液和纳米银悬浮液为基准的评估中的影响。当考虑到测量的沉降分数时，细胞活力的降低取决于沉积在细胞上的 SiO2 纳米颗粒的表面，而与初级颗粒的尺寸无关。这项研究定量地证明了纳米颗粒沉降对于广泛使用的气相二氧化硅体外研究的重要性，并强调沉降测量与倒立直立细胞培养相结合可以成为纳米毒理学研究中的有用工具。
The in vitro cytotoxicity of nanomaterials is commonly assessed by monitoring cell viability under exposure to their suspensions. The actual nanoparticle dose delivered to cells for the same nominal nanoparticle concentration may depend on their settling rate. The goal here is to evaluate quantitatively the importance of settling for the in vitro cytotoxicity evaluation of aerosol-made, fractal-like SiO2 nanoparticles that have similar agglomerate size and structure but varying primary particle diameter. Their deposition rate is measured by UV-visible spectroscopy and their in vitro cytotoxicity is evaluated in both standard upright and inverted murine macrophage culture configurations. Silicas of larger primary particles settle faster and appear more cytotoxic than those of smaller ones in standard upright cell cultures. In contrast, with inverted cell cultures all silicas show similar and much lower cytotoxicity revealing the impact of nanoparticle settling in such assessments that are benchmarked with H2O2 solutions and nanosilver suspensions. When the measured settled fractions are accounted for, the reduction in cell viability depends on the surface of SiO2 nanoparticles deposited onto cells regardless of primary particle size. This study quantitatively demonstrates the importance of nanoparticle settling for in vitro studies of the widely used fumed SiO2 and highlights that settling measurements combined with inverted-upright cell cultures can be useful tools in nanotoxicology studies.