ZnO Nanorods Based Enzymatic Biosensor for Selective Determination of Penicillin.
ZnO Nanorods Based Enzymatic Biosensor for Selective Determination of Penicillin.
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DOI：
10.3390/bios1040153
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发表时间：
2011-10-27
期刊：
影响因子：
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通讯作者：
Willander M
中科院分区：
文献类型：
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作者：
Ibupoto ZH;Ali SM;Khun K;Chey CO;Nur O;Willander M
在这项研究中，我们成功地展示了一种基于排列良好的单晶氧化锌（ZnO）纳米棒的生物传感器的制造，这种纳米棒是用低温水化学生长（ACG）方法生长在镀金玻璃基板上的。以n -5-叠氮-2-硝基苯甲酰氧基琥珀酰亚胺（ANB-NOS）为交联分子，采用青霉酶物理吸附法固定化氧化锌纳米棒。传感器配置的电位响应在100µM到100 mM的大对数浓度范围内显示出良好的线性关系。在研究过程中，该传感器显示出良好的稳定性，对青霉素的灵敏度高达~121 mV/ 10年。电化学反应快速，反应时间小于5 s，对Na1+、K1+、d-葡萄糖、l-葡萄糖、抗坏血酸、尿酸、尿素、蔗糖、乳糖、甘氨酸、青霉素酸和头孢菌素等常见干扰物的反应可忽略。
In this study, we have successfully demonstrated the fabrication of a biosensor based on well aligned single-crystal zinc oxide (ZnO) nanorods which were grown on gold coated glass substrate using a low temperature aqueous chemical growth (ACG) method. The ZnO nanorods were immobilized with penicillinase enzyme using the physical adsorption approach in combination with N-5-azido-2-nitrobenzoyloxysuccinimide (ANB-NOS) as cross linking molecules. The potentiometric response of the sensor configuration revealed good linearity over a large logarithmic concentration range from 100 µM to 100 mM. During the investigations, the proposed sensor showed a good stability with high sensitivity of ~121 mV/decade for sensing of penicillin. A quick electrochemical response of less than 5 s with a good selectivity, repeatability, reproducibility and a negligible response to common interferents such as Na1+, K1+, d-glucose, l-glucose, ascorbic acid, uric acid, urea, sucrose, lactose, glycine, penicilloic acid and cephalosporins, was observed.