COMPUTER-BASED TRACKING OF LIVING CELLS
COMPUTER-BASED TRACKING OF LIVING CELLS
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DOI：
10.1016/0014-4827(82)90414-1
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发表时间：
1982-01-01
影响因子：
3.7
通讯作者：
BERNS, MW
中科院分区：
文献类型：
--
作者：
BERNS, GS;BERNS, MW
一种基于计算机的跟踪技术被开发出来，用于跟踪活细胞的运动，并使它们保持在光学显微镜视野的中心。通过使用图像处理系统，可以充分处理细胞的视频图像以允许计算机识别细胞边界。细胞中心位置的确定使得能够比较连续的细胞位置并校正任何细胞移动。为了说明该技术的多功能性，获得了癌细胞和非癌细胞[3T3和3T12]的运动模式，以确定2种细胞类型之间的运动差异。在1小时内，两种细胞类型之间的运动性没有差异。
A computer-based tracking technique was developed to follow the movement of living cells and keep them centered in the field of view of an optical microscope. With the use of an image-processing system, the video image of a cell can be sufficiently processed to allow computer-recognition of the cell boundaries. Determination of the location of the center of the cell enables comparison of successive cell positions and correction for any cell movement. In order to illustrate the versatility of this technique, patterns of movement were obtained of cancerous and non-cancerous cells [3T3 and 3T12] in an effort to determine the difference in motility between the 2 cell types. There is no difference in the motility between the 2 cell types over 1-h periods.