Narrow Stimulated Resonance Raman Scattering and WGM Lasing in Small Conjugated Polymer Particles for Live Cell Tagging and Tracking
Narrow Stimulated Resonance Raman Scattering and WGM Lasing in Small Conjugated Polymer Particles for Live Cell Tagging and Tracking
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DOI：
10.1002/adom.202001553
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发表时间：
2020-12-09
影响因子：
9
通讯作者：
Kuehne, Alexander J. C.
中科院分区：
文献类型：
--
作者：
Haehnle, Bastian;Lamla, Markus;Kuehne, Alexander J. C.
共轭聚合物颗粒是用于活细胞成像的明亮荧光和稳定的材料。共轭聚合物与回音壁模式（WGM）谐振腔的组合允许从微尺度颗粒发射激光。一旦被细胞内化，激光发射的模式模式就可以用于标记和跟踪，因为每个激光光谱代表一个条形码来识别单个细胞。然而，目前这些颗粒系统受到其大尺寸的限制，这可能会干扰细胞功能。在这里，受激共振拉曼散射（SRRS）在小的共轭聚合物微粒作为一种新的方法，用于产生窄发射作为一种替代WGM为基础的激光发射。这为活细胞的多路光学读出和荧光成像开辟了光谱范围。讨论了单分散微米级聚（芴-二乙烯基苯）粒子的合成，并对其WGM和SRRS发射进行了表征。最后，展示了这些粒子及其发射如何用于活细胞成像和标记。
Conjugated polymer particles are brightly fluorescing and stable materials for live cell imaging. Combination of conjugated polymers with a whispering gallery mode (WGM) resonator allows laser emission from microscale particles. Once internalized by cells, the mode pattern of the laser emission can be used for tagging and tracking, as each laser spectrum represents a bar code to identify individual cells. However, currently these particle systems are limited by their large size, which might interfere with cellular functions. Here, stimulated resonance Raman scattering (SRRS) in small conjugated polymer microparticles is presented as a new method for generating narrow emission as an alternative to WGM-based laser emission. This opens up spectral range for multiplexing optical readout and multicolor imaging of live cells. The synthesis of monodisperse micrometer-sized poly(fluorene-co-divinylbenzene) particles is discussed and their WGM and SRRS emission are characterized. Finally, how these particles and their emission can be employed in live cell imaging and tagging is showcased.