Suppression of Lipopolysaccharide-stimulated Cytokine/Chemokine Production in Skin Cells by Sandalwood Oils and Purified -santalol and -santalol
Suppression of Lipopolysaccharide-stimulated Cytokine/Chemokine Production in Skin Cells by Sandalwood Oils and Purified -santalol and -santalol
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DOI：
10.1002/ptr.5080
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发表时间：
2014-06-01
影响因子：
7.2
通讯作者：
Cox, M. E.
中科院分区：
文献类型：
--
作者：
Sharma, M.;Levenson, C.;Cox, M. E.
在医学上，檀香油（SO）被认为具有抗炎特性;然而，这种活性的机制尚未阐明。为了研究SO如何影响炎症，细胞因子抗体阵列和酶联免疫吸附试验被用来评估的变化，在生产的细胞因子和趋化因子的共培养的人皮肤成纤维细胞和新表皮角质形成细胞暴露于脂多糖和SO从西澳大利亚和东印度檀香树或主要SO组件，檀香醇和檀香醇。脂多糖刺激释放的26种细胞因子和趋化因子，其中20种基本上被同时暴露于两种檀香精油和布洛芬抑制。东印度SO的活性增加与檀香醇浓度增加相关。纯化的-檀香醇和-檀香醇等效地抑制五种指示细胞因子/趋化因子的产生，其浓度与油的檀香醇浓度成比例。纯化的檀香醇和檀香醇还抑制脂多糖诱导的花生四烯酸代谢产物，前列腺素E2和血栓素B2的产生，由皮肤细胞共培养。SO模拟布洛芬非甾体类抗炎药（通过抑制环加氧酶发挥作用）的能力表明了观察到的局部应用SO抗炎特性的可能机制，并为在需要抗炎作用的产品中使用提供了依据。版权所有（c）2013约翰威利父子有限公司
Medicinally, sandalwood oil (SO) has been attributed with antiinflammatory properties; however, mechanism(s) for this activity have not been elucidated. To examine how SOs affect inflammation, cytokine antibody arrays and enzyme-linked immunosorbent assays were used to assess changes in production of cytokines and chemokines by co-cultured human dermal fibroblasts and neo-epidermal keratinocytes exposed to lipopolysaccharides and SOs from Western Australian and East Indian sandalwood trees or to the primary SO components, -santalol and -santalol. Lipopolysaccharides stimulated the release of 26 cytokines and chemokines, 20 of which were substantially suppressed by simultaneous exposure to either of the two sandalwood essential oils and to ibuprofen. The increased activity of East Indian SO correlated with increased santalol concentrations. Purified -santalol and -santalol equivalently suppressed production of five indicator cytokines/chemokines at concentrations proportional to the santalol concentrations of the oils. Purified -santalol and -santalol also suppressed lipopolysaccharide-induced production of the arachidonic acid metabolites, prostaglandin E2, and thromboxane B2, by the skin cell co-cultures. The ability of SOs to mimic ibuprofen non-steroidal antiinflammatory drugs that act by inhibiting cyclooxygenases suggests a possible mechanism for the observed antiinflammatory properties of topically applied SOs and provides a rationale for use in products requiring antiinflammatory effects. Copyright (c) 2013 John Wiley & Sons, Ltd.