Interaction of pyrrolizine derivatives with bovine serum albumin by fluorescence and UV-Vis spectroscopy.
Interaction of pyrrolizine derivatives with bovine serum albumin by fluorescence and UV-Vis spectroscopy.
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DOI：
10.1016/j.saa.2012.05.013
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发表时间：
2012-10
期刊：
影响因子：
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通讯作者：
Jun Zhang;Daxing Xiong;Lingnan Chen;Qilong Kang;Birong Zeng
中科院分区：
文献类型：
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作者：
Jun Zhang;Daxing Xiong;Lingnan Chen;Qilong Kang;Birong Zeng
在模拟生理条件下，用荧光光谱和紫外光谱研究了吡咯嗪衍生物（PD）与牛血清白蛋白（BSA）的相互作用。在302、306和310K三个温度下进行的实验结果表明，PD通过联合猝灭过程引起BSA的荧光猝灭。测定了不同温度下PD与BSA的结合常数（Ka）、结合位点数（n）。根据Förster非辐射能量转移理论，计算了BSA与PD的结合距离r。并得到了相应的热力学参数（ΔG、ΔH和ΔS）。通过比较PD与BSA的结合能力，发现苯环上的取代基可以提高PD与BSA的结合能力。最后，我们通过置换实验研究了BSA上可能与PD结合的亚结构域。
The interaction between pyrrolizine derivatives (PD) and bovine serum albumin (BSA) under imitated physiological conditions was analyzed by fluorescence and ultraviolet spectra. The experiments were conducted at three different temperatures (302, 306 and 310K) and the results showed that PD caused the fluorescence quenching of BSA through a combined quenching procedure. The binding constant (Ka), binding-site number (n) between PD and BSA at different temperatures were obtained. According to Förster non-radiation energy transfer theory, the binding distance (r) between BSA and PD was calculated. The corresponding thermodynamic parameters (ΔG, ΔH, and ΔS) were also obtained. The comparison of binding potency of PD and BSA suggested that the substituent on the benzene ring could enhance the binding affinity of PD and BSA. Finally, we investigated the possible sub-domain on BSA where bind PD by displacement experiments.