Endocannabinoid system in Xenopus laevis development:: CB1 receptor dynamics
Endocannabinoid system in Xenopus laevis development:: CB1 receptor dynamics
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DOI：
10.1016/j.febslet.2006.02.057
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发表时间：
2006-04-03
期刊：
影响因子：
3.5
通讯作者：
Oliana, C
中科院分区：
文献类型：
--
作者：
Beatrice, M;Gabriella, M;Oliana, C
本文首次研究了低等脊椎动物非洲爪蟾发育过程中内源性大麻素系统的动态变化。我们观察到CBI基因在器官发生期间（+/- 1 dpf, st. 28）开始表达，并在分析的三个后续阶段持续表达。注意力集中在CB1信使的定位上，CB1信使在中枢水平（在romboencephalon和嗅觉placods中）和外周水平（在胃肠道中）在3 dpf (st. 41), 4 dpf （st. 46）和12 dpf （st. 49）被发现。我们还考虑了从st. 41开始发生的CB1蛋白的合成，从这个阶段开始，我们使用细胞传感器微物理测量法测试了受体对anandamide的响应功能。CB1功能随着中央和外周水平的发育而增加。这些数据为进一步分析内源性大麻素在青虫胚胎和幼虫生长过程中的生理作用提供了充分的证据。(c) 2006年欧洲生化学会联合会。Elsevier B.V.版权所有。
This study investigates for the first time the dynamics of endocannabinoid system appearance during low vertebrate Xenopus laevis development. We observed that the CBI gene started to be expressed during the organogenesis period (+/- 1 dpf, st. 28) and expression persisted throughout the three further stages analyzed. Attention was focused on the localization of the CB1 messenger that was found both at the central level (in romboencephalon and in olfactory placods) and at the peripheral level (in the gastrointestinal tract) at 3 dpf (st. 41), 4 dpf (st. 46) and 12 dpf (st. 49). We also considered the synthesis of CB1 protein that occurred from st. 41 onwards and, from this stage, we tested the receptor functionality in response to anandamide using cytosensor microphysiometry. CB1 functionality increased with development at both central and peripheral level. These data provide sufficient evidence to encourage further analysis on endocannabinoid physiological roles during embryonic and larval X. laevis growth. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.