Tunicamycin inhibits NMDA and AMPA receptor responses independently of N-glycosylation
Tunicamycin inhibits NMDA and AMPA receptor responses independently of N-glycosylation
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DOI：
10.1016/s0006-8993(03)02838-5
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发表时间：
2003-07-11
期刊：
影响因子：
2.9
通讯作者：
Nishizaki, T
中科院分区：
文献类型：
--
作者：
Maruo, K;Nagata, T;Nishizaki, T
在一个全细胞膜片钳配置，电流通过N-甲基-D-天冬氨酸（NMDA）和α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）受体通道在培养的大鼠海马神经元进行监测，这些电流被抑制到25%和28%的基础水平，分别与衣霉素（10 μ M），蛋白N-糖基化的抑制剂治疗3分钟。衣霉素（10 μ M）减少的幅度引起的群体尖峰在大鼠海马脑片的齿状回，达到78%的基础水平60分钟后开始治疗，和长时程增强（LTP）的穿孔路径从未在衣霉素的存在下诱导。因此，衣霉素似乎作为NMDA和AMPA受体的调节剂，而不管N-糖基化，从而抑制齿状回中的神经传递和LTP。(C)2003 Elsevier Science B. V.保留所有权利。
In a whole-cell patch-clamp configuration, currents through N-methyl-D-aspartate (NMDA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor channels were monitored in cultured rat hippocampal neurons, and those currents were depressed to 25 and 28% of basal levels, respectively, by 3-min treatment with tunicamycin (10 muM), an inhibitor of protein N-glycosylation. Tunicamycin (10 muM) reduced amplitude of population spikes elicited in the dentate gyrus of rat hippocampal slices, reaching 78% of basal levels 60 min after the beginning of treatment, and long-term potentiation (LTP) of the perforant path was never induced in the presence of tunicamycin. Tunicamycin, thus, appears to serve as a modulator for NMDA and AMPA receptors, regardless of N-glycosylation, thereby inhibiting neurotransmission and LTP in the dentate gyrus. (C) 2003 Elsevier Science B.V. All rights reserved.