Visualization of auxin-mediated transcriptional activation using a common auxin-responsive reporter system in the liverwort Marchantia polymorpha
Visualization of auxin-mediated transcriptional activation using a common auxin-responsive reporter system in the liverwort Marchantia polymorpha
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DOI：
10.1007/s10265-012-0477-7
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发表时间：
2012-09-01
影响因子：
2.8
通讯作者：
Kohchi, Takayuki
中科院分区：
文献类型：
--
作者：
Ishizaki, Kimitsune;Nonomura, Maiko;Kohchi, Takayuki
植物激素生长素在陆地植物的各个发育方面起着关键作用。然而，生长素在非维管植物中的反应和分布却知之甚少。在这项研究中，我们提出了转基因植物的地钱表达uidA（GUS）报告基因的大豆生长素诱导型启动子，ProGH 3的控制下，并使用它来间接监测植物生长素介导的转录激活。携带ProGH 3：GUS的转基因植株以生长素依赖的方式显示GUS活性。在无性繁殖过程中，芽杯底部出现GUS组织化学染色。在配子体-孢子体交界处以及受精后发育的孢子体周围也检测到显著的GUS活性。这些结果表明，生长素的活性在黑木耳配子体和孢子体的发育过程中起着至关重要的作用。多形物，生长素介导的转录激活机制已经建立时，植物出现在陆地环境。
The phytohormone auxin plays a pivotal role in various developmental aspects in land plants. However, little is known of the auxin response and distribution in non-vascular plants. In this study, we made transgenic plants of the liverwort Marchantia polymorpha which express the uidA (GUS) reporter gene under control of the soybean auxin-inducible promoter, ProGH3, and used it to indirectly monitor auxin-mediated transcriptional activation in planta. Transgenic plants carrying ProGH3:GUS showed GUS activity in an auxin-dependent manner. Histochemical GUS staining was observed at the bottom of gemma cups in the process of vegetative propagation. Significant GUS activity was also detected around the gametophyte-sporophyte junction as well as the developing sporophyte after fertilization. These results suggest that the activity of auxin is crucial in both gametophyte and sporophyte development in M. polymorpha, and that the mechanism for auxin-mediated transcriptional activation had already been established when plants emerged on the terrestrial environment.