Retinal damage alters gene expression profile in lacrimal glands of mice
Retinal damage alters gene expression profile in lacrimal glands of mice
复制标题
视网膜损伤改变小鼠泪腺的基因表达谱
DOI：
10.1016/j.jphs.2022.02.007
复制
发表时间：
2022
影响因子：
3.5
通讯作者：
Kashimata Masanori
中科院分区：
文献类型：
--
作者：
Ohno Yuta;Yako Tomohiro;Satoh Keitaro;Nagase Haruna;Shitara Akiko;Hara Hideaki;Kashimata Masanori
早期发现青光眼和老年性黄斑变性(AMD)等视网膜疾病对预防失明非常重要。有报道称青光眼和AMD患者泪液中的某些成分发生了变化，这表明泪液在视网膜疾病筛查中具有潜在的实用价值。我们推测，视网膜损伤可能会改变泪腺的基因表达，从而导致泪液成分的变化。我们通过玻璃体内注射N-甲基-D-天冬氨酸(NMDA)或过度光照造成小鼠视网膜损伤。苏木精-伊红染色显示视网膜受损的动物泪腺无组织学变化。然而，在注射或光照后第3天泪腺的RNA测序显示，NMDA组491个基因(268个上调，223个下调)和光照组531个基因(311个上调，220个下调)的表达发生了变化。进一步的基因集浓缩分析表明，这两种类型的视网膜损伤都激活了泪腺中的免疫系统。这是首次证明视网膜损伤可以改变泪腺中的基因表达，并可能导致一种新的非侵入性筛查方法，用于视网膜疾病的早期检测。
Early detection of such retinal diseases as glaucoma and age-related macular degeneration (AMD) is important to prevent blindness. There have been reports of changes in some components in the tears of glaucoma and AMD patients, suggesting tears' potential usefulness in screening for retinal diseases. We hypothesized that retinal damage might alter gene expression in the lacrimal gland, leading to those changes in tear components. We caused retinal damage in mice by intravitreal injection ofN-methyl-d-aspartate (NMDA) or excessive light exposure. Hematoxylin and eosin staining showed no histological changes in the lacrimal glands of animals whose retinas had been damaged. However, RNA sequencing of lacrimal glands on the 3rd day after NMDA injection or light exposure revealed changes in the expression of 491 genes (268 up-regulated; 223 down-regulated) in the NMDA group and 531 genes (311 up-regulated; 220 down-regulated) in the light group. Further gene-set enrichment analysis indicated that both types of retinal damage activated the immune system in the lacrimal glands. This is the first demonstration that retinal damage can alter gene expression in the lacrimal glands, and it might lead to a novel non-invasive screening method for early detection of retinal diseases.