Recent advances in DNA assembly technologies.

Recent advances in DNA assembly technologies.
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DNA组装技术的最新进展。

DOI:
10.1111/1567-1364.12171
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发表时间:
2015-02
影响因子:
3.2
通讯作者:
Zhao H
Zhao H
中科院分区:
生物学4区
文献类型:
--
作者:
Chao R;Yuan Y;Zhao H

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DNA组装是合成生物学和代谢工程最重要的基础技术之一。自 20 世纪 70 年代初开发限制性消化和连接方法以来,人们投入了大量的精力来开发更好的 DNA 组装方法,这些方法具有更高的效率、保真度和模块化性,以及更简单和更快的实验方案。这篇综述不仅总结了关键的 DNA 组装方法及其近期应用,还将重点介绍组装方案的创新以及自动化 DNA 组装方法面临的挑战。
DNA assembly is one of the most important foundational technologies for synthetic biology and metabolic engineering. Since the development of the restriction digestion and ligation method in the early 1970s, a significant amount of effort has been devoted to developing better DNA assembly methods with higher efficiency, fidelity, and modularity, as well as simpler and faster protocols. This review will not only summarize the key DNA assembly methods and their recent applications, but also highlight the innovations in assembly schemes and the challenges in automating the DNA assembly methods.
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