Recent advances in DNA assembly technologies.
Recent advances in DNA assembly technologies.
复制标题
DNA组装技术的最新进展。
DOI:
10.1111/1567-1364.12171
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发表时间:
2015-02
影响因子:
3.2
通讯作者:
Zhao H
中科院分区:
文献类型:
--
作者:
Chao R;Yuan Y;Zhao H
DNA assembly is one of the most important foundational technologies for synthetic biology and metabolic engineering. Since the development of the restriction digestion and ligation method in the early 1970s, a significant amount of effort has been devoted to developing better DNA assembly methods with higher efficiency, fidelity, and modularity, as well as simpler and faster protocols. This review will not only summarize the key DNA assembly methods and their recent applications, but also highlight the innovations in assembly schemes and the challenges in automating the DNA assembly methods.
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