Downregulation of Runx2 by 1,25-Dihydroxyvitamin D₃ Induces the Transdifferentiation of Osteoblasts to Adipocytes.
Downregulation of Runx2 by 1,25-Dihydroxyvitamin D₃ Induces the Transdifferentiation of Osteoblasts to Adipocytes.
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DOI：
10.3390/ijms17050770
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发表时间：
2016-05-19
影响因子：
5.6
通讯作者：
Kim N
中科院分区：
文献类型：
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作者：
Kim JH;Seong S;Kim K;Kim I;Jeong BC;Kim N
1，25-二羟基维生素D3（1，25（OH）2D 3）间接刺激骨形成，但对其对骨形成的直接影响知之甚少。在这项研究中，我们观察到，1，25（OH）2D 3促进脂肪细胞分化，但抑制成骨细胞分化过程中的骨生成。1，25（OH）_2D_3对成骨细胞的成脂分化有促进作用。此外，1，25（OH）2D 3可增强成骨细胞中脂肪细胞标志基因的表达，但抑制成骨细胞标志基因的表达。Runx 2过表达可弥补1，25（OH）2D 3对成骨细胞分化的抑制和对脂肪细胞分化的促进作用。我们的结果表明，1，25（OH）2D 3通过下调成骨细胞中的Runx 2来诱导成骨细胞向脂肪细胞的转分化。
1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) indirectly stimulates bone formation, but little is known about its direct effect on bone formation. In this study, we observed that 1,25(OH)2D3 enhances adipocyte differentiation, but inhibits osteoblast differentiation during osteogenesis. The positive role of 1,25(OH)2D3 in adipocyte differentiation was confirmed when murine osteoblasts were cultured in adipogenic medium. Additionally, 1,25(OH)2D3 enhanced the expression of adipocyte marker genes, but inhibited the expression of osteoblast marker genes in osteoblasts. The inhibition of osteoblast differentiation and promotion of adipocyte differentiation mediated by 1,25(OH)2D3 were compensated by Runx2 overexpression. Our results suggest that 1,25(OH)2D3 induces the transdifferentiation of osteoblasts to adipocytes via Runx2 downregulation in osteoblasts.