Detection of Oxidative Damages on Viral Capsid Protein for Evaluating Structural Integrity and Infectivity of Human Norovirus
Detection of Oxidative Damages on Viral Capsid Protein for Evaluating Structural Integrity and Infectivity of Human Norovirus
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DOI：
10.1021/es9018964
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发表时间：
2010-01-15
影响因子：
11.4
通讯作者：
Bosch, Albert
中科院分区：
文献类型：
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作者：
Sano, Daisuke;Pinto, Rosa M.;Bosch, Albert
人类诺如病毒等不可培养病毒的感染性评价对于确保水和海产品的使用安全至关重要。在这项工作中，我们测试了一种新的方法来评估病毒颗粒的完整性，其中病毒衣壳蛋白上氧化产生的羰基被定量检测。结果，人星状病毒（一种代表性肠道病毒）感染性的降低与病毒颗粒上的氧化损伤量呈正相关。此外，当人诺如病毒处理1 ppm的游离氯15分钟，49.93%的病毒粒子被回收的氧化损伤的颗粒，这是一个5倍的增加，比那些处理0.5 ppm的游离氯15分钟。检测的羰基化的病毒颗粒可能是一个强大的工具，用于评估的减少在noncultureable病毒的感染性。
The infectivity evaluation of noncultivatable viruses, such as human norovirus, is crucial to address needs for ensuring the safety in usage of water and marine products. In this work, we tested a new approach to Evaluate viral particle integrity, in which oxidatively produced carbonyl groups on viral capsid protein were quantitatively detected. As a result the decrease in the infectivity of human astrovirus, a representative enteric virus, positively correlated with the amount of oxidative damage on viral particles. Furthermore, when human norovirus was treated by 1 ppm free chlorine for 15 min, 49.93% of virions were recovered as oxidatively damaged particles, which represents a 5-fold increase over those treated by 0.5 ppm free chlorine for 15 min. The detection of the carbonylated viral particles could be a powerful tool for the evaluation of the decrease in the infectivity of noncultivatable viruses.