In situ detection of Bacillus anthracis spores using fully submersible, self-exciting, self-sensing PMN-PT/Sn piezoelectric microcantilevers
In situ detection of Bacillus anthracis spores using fully submersible, self-exciting, self-sensing PMN-PT/Sn piezoelectric microcantilevers
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DOI：
10.1039/b704579d
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发表时间：
2007-01-01
期刊：
影响因子：
4.2
通讯作者：
Shih, Wei-Heng
中科院分区：
文献类型：
--
作者：
McGovern, John-Paul;Shih, Wan Y.;Shih, Wei-Heng
在这项研究中，我们使用铌镁酸铅-钛酸铅/锡（PMN-PT/Sn）压电微悬臂梁传感器（PEMS）对炭疽杆菌（BA）孢子进行了原位全电检测，该传感器由PMN-PT独立膜制成，并与甲基三甲氧基硅烷（MTMS）电绝缘锡表面上的涂层。将BA孢子表面抗原特异性抗体固定在PMN-PT层的铂电极上。在磷酸盐缓冲盐水（PBS）溶液中，PMN-PT/Sn PEMS表现出50至75的质量（Q）值。在闭环流动池中进行检测，液体体积为0.8 ml，流速为1 ml min（-1）。结果表明，一个传感器“PEMS-A”（500 μ m长，800 μ m宽，具有22 μ m厚的PMN-PT层，20 μ m厚的锡层和1 ± 0.5 × 10（-12）g Hz（-1）质量检测灵敏度）显示出2100 ± 200的共振频率偏移，浓度分别为20 000、2000和200个孢子ml（-1）或16 000、1600和160个总孢子时，1100 +/- 100和700 +/- 100 Hz。此外，“`PEMS-B”（350 μ m长，800 μ m宽，具有8 μ m厚的PMN-PT层，6 μ m厚的锡层和2 +/- 1 × 10（-13）g Hz（-1）质量检测灵敏度）显示出2400 +/- 200，1500 +/- 200，浓度分别为20 000、2000、100和45个孢子ml（-1）或16 000、1600、80和36个孢子时，500 +/- 150和200 +/- 100 Hz。
In this study, we have demonstrated in situ, all-electrical detection of Bacillus anthracis ( BA) spores using lead magnesium niobate-lead titanate/tin (PMN-PT/Sn) piezoelectric microcantilever sensors ( PEMS) fabricated from PMN-PT freestanding films and electrically insulated with methyltrimethoxysilane (MTMS) coatings on the tin surface. Antibody specific to BA spore surface antigen was immobilized on the platinum electrode of the PMN-PT layer. In phosphate-buffered saline (PBS) solution, the PMN-PT/Sn PEMS exhibited quality (Q) values ranging from 50 to 75. The detection was carried out in a closed-loop flow cell with a liquid volume of 0.8 ml and a flow rate of 1 ml min(-1). It was shown that one sensor, "PEMS-A'' ( 500 mu m long, 800 mu m wide, with a 22 mu m thick PMN-PT layer, a 20 mu m thick tin layer and a 1 +/- 0.5 x 10(-12) g Hz(-1) mass detection sensitivity) exhibited resonance frequency shifts of 2100 +/- 200, 1100 +/- 100 and 700 +/- 100 Hz at concentrations of 20 000, 2000, and 200 spores ml(-1) or 16 000, 1600, and 160 total spores, respectively. Additionally, "`PEMS-B'' ( 350 mu m long, 800 mu m wide, with an 8 mu m thick PMN-PT layer, a 6 mu m thick tin layer and a 2 +/- 1 x 10(-13) g Hz(-1) mass detection sensitivity) exhibited resonance frequency shifts of 2400 +/- 200, 1500 +/- 200, 500 +/- 150 and 200 +/- 100 Hz at concentrations of 20 000, 2000, 100, and 45 spores ml(-1) or 16 000, 1600, 80, and 36 total spores, respectively.