The GGAs promote ARF-dependent recruitment of clathrin to the TGN
The GGAs promote ARF-dependent recruitment of clathrin to the TGN
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DOI：
10.1016/s0092-8674(01)00299-9
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发表时间：
2001-04-06
期刊：
影响因子：
64.5
通讯作者：
Bonifacino, JS
中科院分区：
文献类型：
--
作者：
Puertollano, R;Randazzo, PA;Bonifacino, JS
The GGAs constitute a family of modular adaptor-related proteins that bind ADP-ribosylation factors (ARFs) and localize to the trans-Golgi network (TGN) via their GAT domains. Here, we show that binding of the GAT domain stabilizes membrane-bound ARF1 . GTP due to interference with the action of GTPase-activating proteins. We also show that the hinge and ear domains of the GGAs interact with clathrin in vitro, and that the GGAs promote recruitment of clathrin to liposomes in vitro and to TGN membranes in vivo. These observations suggest that the GGAs could function to link clathrin to membrane-bound ARF GTP.
