[Metabonomics Study on Urine 1H-NMR in Chronic Superficial Gastritis Patients with Pi-qi Deficiency Syndrome/Pi-Wei Dampness-heat Syndrome].
[Metabonomics Study on Urine 1H-NMR in Chronic Superficial Gastritis Patients with Pi-qi Deficiency Syndrome/Pi-Wei Dampness-heat Syndrome].
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发表时间：
2015-12
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通讯作者：
Xu-Guang Shi;Zhongjie Zou;Meigui Wu;Yuan-gui Zeng;Z. Lian;Manting Huang;Mengjuan Gong
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作者：
Xu-Guang Shi;Zhongjie Zou;Meigui Wu;Yuan-gui Zeng;Z. Lian;Manting Huang;Mengjuan Gong
目的观察慢性浅表性胃炎（CSG）脾气虚证（PQDS）或脾胃湿热证（PWDHS）患者尿液代谢组的变化，为其辨证提供科学依据。方法采集CSG PQDS/PWDHS患者和健康志愿者的尿液样本，每组10例。对尿液样本进行基于质子核磁共振波谱 (1H-NMR) 的代谢组学分析。通过主成分分析（PCA）、偏最小二乘判别分析（PLS-DA）和尿变量统计分析对相关生物标志物的含量进行分析。结果 PLS-DA分析显示CSG PQDS/PWDHS患者和健康志愿者的代谢物可以相互区分。从患有 PQDS 的 CSG 患者和健康志愿者的尿液中筛选出 7 种差异鉴定的代谢物，包括谷氨酸、蛋氨酸、α-酮戊二酸、二甲基甘氨酸、肌酐、牛磺酸和葡萄糖。从患有 PWDHS 的 CSG 患者和健康志愿者的尿液中筛选出四种差异鉴定的代谢物，包括 2-羟基丁酸、三甲胺氧化物、牛磺酸和马尿酸。从患有 PQDS 和 PWDHS 的 CSG 患者的尿液中筛选出 11 种差异鉴定的代谢物，包括岩藻糖、β-羟基丁酸、丙氨酸、谷氨酸、蛋氨酸、琥珀酸、柠檬酸、肌酐、葡萄糖、马尿酸和乳酸。结论 CSG患者PQDS和PWDHS的代谢差异主要表现在糖代谢、脂质代谢和氨基酸分解代谢上，基于1H-NMR的代谢组学可用于中医证候分型的分类研究。
OBJECTIVE To observe metabolomic changes in urine of chronic superficial gastritis (CSG) patients with Pi-qi deficiency syndrome (PQDS) or Pi-Wei dampness-heat syndrome (PWDHS), thereby providing scientific evidence for syndrome typing of them. METHODS Urine samples were collected from CSG patients with PQDS/PWDHS and healthy volunteers, 10 in each group. Proton nuclear magnetic resonance spectroscopy (1H-NMR) based metabonomic analysis was performed on urine samples. Contents of related biomarkers were analyzed by principal component analysis (PCA), partial least square discriminant analysis (PLS-DA), and urivariate statistical analysis. RESULTS PLS-DA analysis showed that metabolites among CSG patients with PQDS/PWDHS and healthy volunteers could be mutually distinguished. Seven differentially identified metabolites were screened from urines of CSG patients with PQDS and healthy volunteers included glutamate, methionine, α-oxoglutarate, dimethylglycine, creatinine, taurine, and glucose. Four differentially identified metabolites were screened from urines of CSG patients with PWDHS and healthy volunteers included 2-hydroxybutyric acid, trimethylamine oxide, taurine, and hippuric acid. Eleven differentially identified metabolites were screened from urines of CSG patients with PQDS and PWDHS included fucose, β-hydroxybutyric acid, alanine, glutamate, methionine, succinic acid, citric acid, creatinine, glucose, hippuric acid, and lactic acid. CONCLUSION The metabolic differences of CSG patients PQDS and PWDHS mainly manifested in glycometabolism, lipid metabolism, and amino acids catabolism, and 1H-NMR based metabonomics may be used in classified study of Chinese medical syndrome typing.