Oxygen radical‐induced neurotoxicity in spinal cord neuron cultures
Oxygen radical‐induced neurotoxicity in spinal cord neuron cultures
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脊髓神经元培养物中氧自由基诱导的神经毒性
DOI：
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发表时间：
1994
影响因子：
4.2
通讯作者：
Seung U. Kim
中科院分区：
文献类型：
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作者：
M. Michikawa;K. T. Lim;J. McLarnon;Seung U. Kim
氧自由基对胎鼠脊髓神经元培养物的神经毒性作用已有研究。通过在培养基中添加黄嘌呤氧化酶和次黄嘌呤产生氧自由基、超氧自由基和过氧化氢。在脊髓神经元培养物中，神经元暴露于这种氧自由基生成系统会导致细胞显着死亡，胆碱乙酰转移酶（ChAT）活性以时间依赖性方式降低。氧自由基引起的细胞活力和 ChAT 酶活性的降低被超氧化物歧化酶 (SOD)、过氧化氢酶和四(2-吡啶甲基)乙二胺 (TPEN)（一种金属螯合剂）等清除剂阻断。 N-甲基-D-天冬氨酸 (NMDA) 受体的拮抗剂，包括 MK801（一种非竞争性 NMDA 拮抗剂）、D-2-氨基-5-磷酸戊酸 (APV)（一种竞争性 NMDA 拮抗剂）和 7-氯犬尿酸（与 NMDA 受体相关的甘氨酸位点的拮抗剂），同样可以阻止氧自由基诱导的脊髓神经元培养物中细胞活力和 ChAT 活性的下降。这些结果表明氧自由基和兴奋性毒性氨基酸都参与了脊髓神经元的氧化剂引发的神经毒性。 © 1994 Wiley-Liss, Inc.
The neurotoxic effects of oxygen radical on spinal cord neuron cultures derived from fetal mouse have been studies. Oxygen radicals, superoxide radical and hydrogen peroxide, were generated by adding xanthine oxidase and hypoxanthine in the culture medium. Exposure of neurons to this oxygen radical generating system resulted in a significant cell death and decrease of choline acetyltransferase (ChAT) activity in a time‐dependent manner in spinal cord neuron cultures. The decrease in cell viability and ChAT enzyme activity induced by the oxygen radicals was blocked by scavengers such as superoxide dismutase (SOD), catalase, and tetrakis (2‐pyridylmethyl) ethylenediamine (TPEN), a metal chelator. Antagonista of the N‐methyl‐D‐aspartate (NMDA) receptor, including MK801 (a noncompetitive NMDA antagonist), D‐2‐amino‐5‐phosphonovaleric acid (APV) (a competitive NMDA antagonist), and 7‐chlorokynurenic acid (an antagonist at the glycine site associated with the NMDA receptor), similarly blocked oxygen radical‐induced decrease in cell viability and ChAT activity in spinal cord neuron cultures. These results indicate that both oxygen radicals and excitotoxic amino acids were involved in the oxidant‐ititiated neurotoxicity of spinal cord neurons. © 1994 Wiley‐Liss, Inc.
肌萎缩侧索硬化症中的谷氨酸功能障碍和选择性运动神经元变性：一种假设。
影响因子：
11.2
作者：
Plaitakis,A
通讯作者：
Plaitakis,A