An E alpha E beta heterodimer dramatically alters selection of functional T-cell subsets in A.CA transgenic mice.
An E alpha E beta heterodimer dramatically alters selection of functional T-cell subsets in A.CA transgenic mice.
复制标题
E α E β 异二聚体极大地改变了 A.CA 转基因小鼠中功能性 T 细胞亚群的选择。
DOI：
10.1007/bf00188808
复制
发表时间：
1993
期刊：
影响因子：
3.2
通讯作者：
Diamond,B
中科院分区：
文献类型：
--
作者：
Ishikawa,S;Kowal,C;Diamond,B
通过构建表达MHC编码的ed分子α和β链的转基因小鼠，比较e- α - a - β混合同型异源二聚体和e- α - a - β同型异源二聚体在胸腺选择中的作用。双转基因e - α - e β小鼠的CD4+T细胞数量明显高于单转基因e - α - a β小鼠。CD4+、CD8+Tcrb (T细胞受体b)- v6 +、Tcrb- v7 +、Tcrb- v10 +T细胞仅在ineaeb双转基因小鼠中被阳性选择，而CD8+Tcrb- v5 +T细胞在单转基因和双转基因小鼠中均被阴性选择。
Transgenic mice expressing the α and β chains of the Edmolecule encoded by the MHC were generated to investigate the role of thymic selection of EαEβ isotype-matched heterodimers compared with EαAβ mixed-isotype heterodimers. Double transgenic mice, which expressed EαEβ molecules, showed a marked increase in CD4+T cells compared withEa-only transgenic mice expressing EαAβ molecules. CD4+, but not CD8+Tcrb (T-cell receptor b)-V6+, Tcrb-V7+, and Tcrb-V10+T cell were positively selected only inEaEbdouble transgenic mice, while CD8+Tcrb-V5+T cells were negatively selected in both single- and double-transgenic mice.