Analysis of the miRNA transcriptome during testicular development and spermatogenesis of the Mongolian horse
Analysis of the miRNA transcriptome during testicular development and spermatogenesis of the Mongolian horse
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蒙古马睾丸发育和精子发生过程中miRNA转录组分析
DOI：
10.1071/rd19133
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发表时间：
2020-01-01
影响因子：
1.9
通讯作者：
Manglai, Dugarjaviin
中科院分区：
文献类型：
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作者：
Li Bei;He Xiaolong;Manglai, Dugarjaviin
大量研究表明，microRNAs(MiRNAs)对睾丸发育和精子发生是必不可少的。为了进一步研究这些生理过程，收集了蒙古马三个未成熟和三个成熟的精巢，并建立了6个文库。利用小RNA测序技术，共鉴定出531个成熟miRNAs，其中46个为新发现的未发现功能的miRNAs。在531个miRNAs中，421个在未成熟文库和成熟文库中均有表达，65个miRNAs仅在未成熟睾丸文库中表达，45个miRNAs仅在成熟睾丸文库中表达。此外，在未成熟文库和成熟文库中发现的miRNAs中，有107个存在显著差异表达(校正P值(Padj)和t；0.05)。在仅在未成熟睾丸中表达的miRNAs中，有2个miRNAs差异表达，而在仅在成熟睾丸中表达的miRNAs中，有9个miRNAs差异表达。对miRNA和mRNA表达谱的综合分析预测了107个miRNA-mRNA相互作用位点。基因本体论(GO)和京都基因与基因组百科全书(KEGG)对预测的靶基因进行的路径分析表明，差异表达的miRNAs在睾丸发育和精子发生中发挥作用。这些发现确定miRNAs是蒙古马睾丸发育和精子发生的关键因素，这也可能有助于我们理解相关哺乳动物物种的生育机制。
Numerous studies have shown that microRNAs (miRNAs) are essential for testicular development and spermatogenesis. In order to further characterise these physiological processes, three immature and three mature testes of the Mongolian horse were collected and six libraries were established. Using small RNA sequencing technology, 531 mature miRNAs were identified, including 46 novel miRNAs without previously ascribed functions. Among the 531 miRNAs, 421 were expressed in both immature and mature libraries, 65 miRNAs were found solely in immature testis libraries and 45 miRNAs were found solely in mature testis libraries. Furthermore, among the miRNAs that were identified in both immature and mature libraries, 107 were significantly differentially expressed (corrected P value (padj) < 0.05). Among the miRNAs that were only expressed in immature testes, two miRNAs were differentially expressed, whereas among the miRNAs that were only expressed in mature testes, nine miRNAs were differentially expressed. Comprehensive analysis of miRNA and mRNA expression profiles predicted 107 miRNA-mRNA interaction sites. Gene ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis of the predicted target genes suggested roles of the differentially expressed miRNAs in testicular development and spermatogenesis. These findings identify miRNAs as key factors in the development of the testes and spermatogenesis in the Mongolian horse, which may also help us to understand the mechanisms of fertility in related mammalian species.