Perivascular clusters of dendritic cells provide critical survival signals to B cells in bone marrow niches
Perivascular clusters of dendritic cells provide critical survival signals to B cells in bone marrow niches
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DOI：
10.1038/ni1571
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发表时间：
2008-04-01
影响因子：
30.5
通讯作者：
Jung, Steffen
中科院分区：
文献类型：
--
作者：
Sapoznikov, Anita;Pewzner-Jung, Yael;Jung, Steffen
除了其既定的造血功能外，骨髓还拥有成熟的淋巴细胞，并在启动T细胞和B细胞反应中充当次级淋巴器官。在这里，我们报告了骨髓树突状细胞(BmDC)的特征。多光子成像显示，BmDC被组织成包裹血管的血管周围簇，并种植有成熟的B淋巴细胞和T淋巴细胞。有条件地去除这些骨髓免疫壁龛中的bmDC会导致成熟B细胞的特异性丧失，这种表型可以通过B细胞中抗凋亡因子Bcl-2的过度表达而逆转。BmDC的存在通过产生巨噬细胞移动抑制因子促进了循环B细胞在骨髓中的存活。因此，bmDC对于维持骨髓中循环的B细胞至关重要。
Beyond its established function in hematopoiesis, the bone marrow hosts mature lymphocytes and acts as a secondary lymphoid organ in the initiation of T cell and B cell responses. Here we report the characterization of bone marrow-resident dendritic cells (bmDCs). Multiphoton imaging showed that bmDCs were organized into perivascular clusters that enveloped blood vessels and were seeded with mature B lymphocytes and T lymphocytes. Conditional ablation of bmDCs in these bone marrow immune niches led to the specific loss of mature B cells, a phenotype that could be reversed by overexpression of the antiapoptotic factor Bcl-2 in B cells. The presence of bmDCs promoted the survival of recirculating B cells in the bone marrow through the production of macrophage migration-inhibitory factor. Thus, bmDCs are critical for the maintenance of recirculating B cells in the bone marrow.