Immune-deficient Drosophila melanogaster:: A model for the innate immune response to human fungal pathogens
Immune-deficient Drosophila melanogaster:: A model for the innate immune response to human fungal pathogens
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DOI：
10.4049/jimmunol.172.9.5622
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发表时间：
2004-05-01
影响因子：
4.4
通讯作者：
Whiteway, M
中科院分区：
文献类型：
--
作者：
Alarco, AM;Marcil, A;Whiteway, M
我们探讨了宿主-病原体相互作用的人类机会致病真菌白色念珠菌使用果蝇。我们建立了一个果蝇菌株缺乏功能Toll受体是高度敏感的人类病原体白色念珠菌。利用这种敏感菌株，我们已经能够证明，一组特定的C。在哺乳动物感染模型中不同毒力的白念珠菌突变体在果蝇中的毒力也受到损害，并且显著地显示出相同的毒力等级顺序。该免疫缺陷昆虫模型还揭示了在免疫活性小鼠感染模型中未检测到的毒力特性。宿主和病原体中的遗传系统将使宿主特异性组分和参与宿主-真菌相互作用的白色念珠菌基因的鉴定成为可能。
We explored the host-pathogen interactions of the human opportunistic fungus Candida albicans using Drosophila melanogaster. We established that a Drosophila strain devoid of functional Toll receptor is highly susceptible to the human pathogen C albicans. Using this sensitive strain, we have been able to show that a set of specific C. albicans mutants of different virulence in mammalian infection models are also impaired in virulence in Drosophila and remarkably display the same rank order of virulence. This immunodeficient insect model also revealed virulence properties undetected in an immunocompetent murine model of infection. The genetic systems available in both host and pathogen will enable the identification of host-specific components and C albicans genes involved in the host-fungal interplay.