Photon counting histogram for one-photon excitation
Photon counting histogram for one-photon excitation
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DOI：
10.1002/cphc.200300824
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发表时间：
2003-10-17
期刊：
影响因子：
2.9
通讯作者：
Zare, RN
中科院分区：
文献类型：
--
作者：
Perroud, TD;Huang, B;Zare, RN
Poisson（k，x）xke x k！(2)是泊松分布，它描述了当接收到的平均光子数为x时，获得k个光子的概率。遵循Chen等人描述的标准卷积程序。[2]获得光子计数直方图的最终形式，其由每种荧光物质的两个参数确定：观察体积中的平均分子数N和分子亮度ε。为了具有与荧光相关光谱法（FCS）中相同的N π，将V0设定为与FCS [3，4][等式（3）]中定义的观察体积成比例：链，即使在水解后臂的聚合度也将保持相同。在此假设下，我们根据C6 H5（6.5±7.8 ppm）和NCH 2CH 2（2.8±3.8 ppm）的积分比计算PEI和PPOZ的嵌段长度;此信息显示在方案1中。
Poisson (k, x) xke Àx k!(2) is the Poisson distribution that describes the probability of getting k photons when the average number of photons received is x. Following the standard convolution procedure described by Chen et al.[2] the final form for the photon counting histogram is obtained, which is determined by two parameters for each fluorescent species: the average number of molecules in the observation volume, N≈, and the molecular brightness, ε. In order to have the same N≈ as in fluorescence correlation spectroscopy (FCS), V0 is set proportional to the observation volume defined in FCS [3, 4][Equation (3)]: chain, the polymerization degree of the arms would remain the same even after the hydrolysis. With this assumption, we calculated the block lengths of PEI and PPOZ from the integration ratio of C6H5 (6.5±7.8 ppm) and NCH2CH2(2.8±3.8 ppm); this information is shown in Scheme 1.