Repair of cranial bone defects with adipose derived stem cells and coral scaffold in a canine model
Repair of cranial bone defects with adipose derived stem cells and coral scaffold in a canine model
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在犬模型中用脂肪干细胞和珊瑚支架修复颅骨缺损
DOI：
10.1016/j.biomaterials.2007.08.042
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发表时间：
2007-12-01
期刊：
影响因子：
14
通讯作者：
Cao, Yilin
中科院分区：
文献类型：
--
作者：
Cui, Lei;Liu, Bo;Cao, Yilin
具有成骨分化潜能的脂肪干细胞（ASC）已被证明是骨再生的替代细胞来源。然而，以前的大多数体内研究都是在小动物身上进行的，沿着时间相对较短。在本研究中，我们探讨了使用ASC和珊瑚支架修复犬颅骨缺损模型的可行性，并随访了长达6个月的结果。将从犬皮下脂肪分离的自体ASC扩增，成骨诱导，并接种在珊瑚支架上。创建双侧顶骨全层缺损（20 mm x 20 mm）。缺损用ASC-珊瑚结构（实验组）或单独用珊瑚（对照组）修复。三维CT扫描显示，实验组在植入后12周有新骨形成，而对照组珊瑚支架部分降解。移植后24周的X线片分析显示，实验侧平均每个缺损体积的84.19 ± 6.45%得到修复，而对照侧仅填充其体积的25.04 ± 18.82%。组织学检查显示，实验侧缺损由典型的骨组织修复，而对照组仅观察到少量骨形成和纤维连接。通过目前的方法成功修复了临界尺寸的骨缺损，证实了使用ASC与珊瑚支架进行骨再生的潜力。(C)2007爱思唯尔有限公司版权所有。
Adipose derived stem cells (ASCs) with osteogenic differentiation potential have been documented as an alternative cell source for bone regeneration. However, most of previous in vivo studies were carried out on small animals along with relatively short-term follow-up. In this study, we investigated the feasibility of using ASCs and coral scaffolds to repair a cranial bone defect in a canine model, and followed up the outcome for up to 6 month. Autologous ASCs isolated from canine subcutaneous fat were expanded, osteogenically induced, and seeded on coral scaffolds. Bilateral full-thickness defects (20 mm x 20 mm) of parietal bone were created. The defects were either repaired with ASC-coral constructs (experimental group) or with coral alone (control group). Three-dimensional CT scan showed that new bones were formed in the experimental group at 12 weeks post-implantation, while coral scaffolds were partially degraded in the control group. By radiographic analysis at 24 weeks post-transplantation, it was shown that an average of 84.19 +/- 6.45% of each defect volume had been repaired in experimental side, while the control side had only 25.04 +/- 18.82% of its volume filled. Histological examination revealed that the defect was repaired by typical bone tissue in experimental side, while only minimal bone formation with fibrous connection was observed in the control group. The successful repair of critical-sized bone defects via the current approach substantiates the potentiality of using ASCs with coral scaffolds for bone regeneration. (C) 2007 Elsevier Ltd. All rights reserved.