A CLASS-I ANTIGEN, HLA-G, EXPRESSED IN HUMAN TROPHOBLASTS
A CLASS-I ANTIGEN, HLA-G, EXPRESSED IN HUMAN TROPHOBLASTS
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DOI：
10.1126/science.2326636
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发表时间：
1990-04-13
期刊：
影响因子：
56.9
通讯作者：
DEMARS, R
中科院分区：
文献类型：
--
作者：
KOVATS, S;MAIN, EK;DEMARS, R
α人类组织相容性抗原HLA-G的链通过使用二维凝胶电泳被鉴定为五种37- 39-千道尔顿同种型的阵列。细胞相关的和分泌的HLA-G抗原在妊娠早期绒毛细胞滋养层细胞中是突出的，并且在妊娠晚期细胞滋养层细胞中大大减少。未检测到等位基因变异，表明HLA-G在细胞滋养层细胞中没有明显的多态性。在所研究的以下绒毛膜癌细胞系中，HLA-G在JEG中表达，而在Jar或BeWo中不表达。内源性HLA-G基因的表达在正常淋巴细胞中尚未发现。因此，HLA-G受到细胞类型特异性和发育调节，并在妊娠早期人类细胞滋养层中表达。
The .alpha. chain of the human histocompatibility antigen HLA-G was identified as an array of five 37- to 39-kilodalton isoforms by the use of two-dimensional gel electrophoresis. Both cell-associated and secreted HLA-G antigens are prominent in first trimester villous cytotrophoblasts and are greatly reduced in third trimester cytotrophoblasts. Allelic variation was not detected, an indication that HLA-G is not obviously polymorphic in cytotrophoblasts. Among the following choriocarcinoma cell lines studied, HLA-G is expressed in JEG but not in Jar or BeWo. Expression of endogenous HLA-G genes has not been found in normal lymphoid cells. Thus, HLA-G is subject to both cell type-specific and developmental regulation and is expressed in early gestation human cytotrophoblasts.