The Development of TALE Nucleases for Biotechnology.
The Development of TALE Nucleases for Biotechnology.
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DOI：
10.1007/978-1-4939-2932-0_3
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Gersbach CA
中科院分区：
文献类型：
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作者：
Ousterout DG;Gersbach CA
基于转录激活因子样效应核酸酶（TALENs）的简易基因组工程技术的发展已经在科学和医学的各个领域取得了重大进展。本文综述了TALENs的发展及其在基础科学、生物技术和生物医学领域的应用。这包括通过TALEs发现DNA识别，设计新的TALE蛋白以适应不同的靶标，基于核酸酶的编辑策略的一般进展，以及针对TALEN技术的各种应用所面临的挑战。我们回顾了应用TALENs研究基因功能和调控、建立疾病模型和开发基因治疗的例子。还讨论了基因组编辑的现状和这些技术的其他用途的未来方向。
The development of a facile genome engineering technology based on transcription activator-like effector nucleases (TALENs) has led to significant advances in diverse areas of science and medicine. In this review, we provide a broad overview of the development of TALENs and the use of this technology in basic science, biotechnology, and biomedical applications. This includes the discovery of DNA recognition by TALEs, engineering new TALE proteins to diverse targets, general advances in nuclease-based editing strategies, and challenges that are specific to various applications of the TALEN technology. We review examples of applying TALENs for studying gene function and regulation, generating disease models, and developing gene therapies. The current status of genome editing and future directions for other uses of these technologies are also discussed.