The hypothermic effect of 5-CT in mice is mediated through the 5-HT7 receptor
The hypothermic effect of 5-CT in mice is mediated through the 5-HT7 receptor
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DOI：
10.1016/s0028-3908(03)00117-5
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发表时间：
2003-06-01
影响因子：
4.7
通讯作者：
Hutson, PH
中科院分区：
文献类型：
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作者：
Guscott, MR;Egan, E;Hutson, PH
5-HT7 受体是 5-HT 受体家族的新成员，迄今为止，对其在中枢神经系统中的潜在作用尚无明确的认识。该受体已通过原位杂交和 5-HT7 样免疫反应性进行定位，并已在大脑的离散区域中检测到。包括下丘脑（Oliver 等，1999）。这表明该受体可能参与温度调节，Hagan 等人。 (2000) 已经表明，选择性 5-HT7 受体拮抗剂可以逆转 5-CT 对豚鼠的低温效应。目前的研究证实，5-HT7 受体拮抗剂 SB-269970（1-30 mg/kg，腹腔注射）和 SB-258719（5-20 mg/kg，腹腔注射），但不是 5-HT7 受体拮抗剂 WAY 100635（0.1-1 mg/kg，皮下注射）或 5-HT1B/D 拮抗剂， GR127935 （1.25-5 mg/kg，腹膜内注射）逆转了 5-CT 对小鼠的低温作用。此外，在 5-HT7 受体无效突变小鼠中检查了 5-CT 对体温的影响。 5-CT（0.1-1 mg/kg，腹腔注射）显着降低野生型小鼠的直肠温度，但不降低 5-HT7 受体敲除小鼠的直肠温度。这表明 5-CT 的低温 1 效应是通过 5-HT7 受体介导的。所有程序均按照英国动物（科学程序）法（1986 年）进行。 (C) 2003 Elsevier Science Ltd. 保留所有权利。
The 5-HT7 receptor is a recent addition to the 5-HT receptor family and to date there is no clear idea as to its potential role in the CNS. The receptor has been mapped by in situ hybridization and 5-HT7-like immunoreactivity and has been detected in discrete areas of the brain. including the hypothalamus (Oliver et al., 1999). This suggests the receptor may be involved in temperature regulation and Hagan et al. (2000) have shown that a selective 5-HT7 receptor antagonist reverses the hypothermic effect of 5-CT in guinea-pigs. The current study confirmed that the 5-HT7 receptor antagonists, SB-269970 (1-30 mg/kg, i.p.) and SB-258719 (5-20 mg/kg, i.p.), but not the 5-HT7 receptor antagonist, WAY 100635(0.1-1 mg/kg, s.c.), or the 5-HT1B/D antagonist, GR127935 (1.25-5 mg/kg, i.p.), reversed the hypothermic effect of 5-CT in mice. In addition the effect of 5-CT on body temperature was examined on 5-HT7 receptor null mutant mice. 5-CT (0.1-1 mg/kg, i.p.) significantly reduced rectal temperature in wildtype but not 5-HT7 receptor knockout mice. This suggests that the hypothermic 1 effects of 5-CT are mediated through the 5-HT7 receptor. All procedures were carried out in accordance with the UK Animals (Scientific Procedures) Act (1986). (C) 2003 Elsevier Science Ltd. All rights reserved.