Particle capture by marine bacteria
Particle capture by marine bacteria
复制标题
海洋细菌捕获颗粒
DOI：
10.3354/ame01147
复制
发表时间：
2007
影响因子：
1.4
通讯作者：
K. Kogure
中科院分区：
文献类型：
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作者：
Y. Seo;Eiko Ikemoto;Akihiro Yoshida;K. Kogure
在水环境中，通常存在大量的亚微米颗粒，它们的周转和归宿在地球化学循环中具有相当重要的生态意义。如果颗粒保留在细菌细胞的表面上而不是保持悬浮在水相中，则可以实现亚微米颗粒的有效降解。我们假设某些细菌群具有颗粒捕获（PC）能力。为了验证这个假设，我们采取了两种方法。首先，在天然海水样品中加入顺磁性颗粒，分离出具有PC能力的细菌群。结合变性梯度凝胶电泳（DGGE）和多维标度（MDS）对分离的细菌进行群落结构分析。用这种新方法回收的细菌群既不同于原始海水样品中的代表性细菌群，也不同于大颗粒相关细菌群。第二，原子力显微镜用于直接观察自然细菌种群中的亚微米颗粒。在东京湾内部和公海500至2000 m深处，保留亚微米颗粒的细胞数量很高。我们的结论是，在天然海水中有细菌群体，具有PC能力，我们成功地收集这些细胞使用新的顺磁性粒子方法在这里提出。
In aquatic environments large numbers of submicron particles are generally present, and their turnover and fate are of considerable ecological importance in geochemical cycles. Efficient degradation of submicron particles can be attained if the particles are retained on the surfaces of bac- terial cells rather than remaining suspended in the aqueous phase. We hypothesized that some bac- terial groups possess particle-capture (PC) ability. To verify this hypothesis, we took 2 approaches. First, paramagnetic particles were added to natural seawater samples to separate out bacterial groups with PC capabilities. Method development was carried out in combination with community structure analysis of the separated bacteria by denaturing gradient gel electrophoresis (DGGE) and multidimensional scaling (MDS). Bacterial groups recovered by this new approach were different from either the representative ones in the original seawater sample or large particle associated bac- terial groups. Second, atomic force microscopy was used to directly observe the submicron particles within natural bacterial populations. The number of cells retaining submicron particles was high in the inner part of Tokyo Bay and at depths of 500 to 2000 m in the open ocean. We concluded that there are bacterial groups in natural seawater that possess PC ability and we successfully collected these cells using the new paramagnetic particle method presented here.
影响因子：
2.3
作者：
Nishino, T;Ikemoto, E;Kogure, K
通讯作者：
Kogure, K
影响因子：
4.4
作者：
MUYZER, G;DEWAAL, EC;UITTERLINDEN, AG
通讯作者：
UITTERLINDEN, AG
DOI：
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