The auxiliary ESCRT complexes provide robustness to cold in poikilothermic organisms
The auxiliary ESCRT complexes provide robustness to cold in poikilothermic organisms
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DOI：
10.1242/bio.043422
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发表时间：
2019-09-01
期刊：
影响因子：
2.4
通讯作者：
Klein, Thomas
中科院分区：
文献类型：
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作者：
Baeumers, Miriam;Klose, Sven;Klein, Thomas
ESCRT途径由ESCRT-0、-I、-II、-III和Vps4复合体依次作用，引导膜脱离细胞质。虽然中央ESCRT-III核心复合体的成分已经被彻底研究，但相关的两个辅助ESCRT亚复合体的成分在后生动物中的功能还不是很清楚，特别是在生物水平上。本文对两个辅助亚复合体中的辅助ESCRTs Chmp5和Ist1、DChmp5和DIst1的同源基因进行了发育分析。每个单零突变体都表现出轻微的发育缺陷，而Dist1Dchmp5双突变体则表现出严重的缺陷，这表明这两个基因是协同作用的，但在不同的途径上。此外，所提出的结果表明，辅助ESCRT-III可能通过阻止ESCRT-III核心成分灌木在胚体膜上的积累，在不同的变温生物的发育过程中提供了对寒冷的稳健性。
The ESCRT pathway, comprising the in sequence acting ESCRT-0, -I, -II, -III and Vps4 complexes, conducts the abscission of membranes away from the cytosol. Whereas the components of the central ESCRT-III core complex have been thoroughly investigated, the function of the components of the associated two auxiliary ESCRT sub-complexes are not well-understood in metazoans, especially at the organismal level. We here present the developmental analysis of the Drosophila orthologs of the auxiliary ESCRTs Chmp5 and Ist1, DChmp5 and DIst1, which belong to the two auxiliary sub-complexes. While each single null mutant displayed mild defects in development, the Dist1 Dchmp5 double mutant displayed a severe defect, indicating that the two genes act synergistically, but in separate pathways. Moreover, the presented results indicate that the auxiliary ESCRTs provide robustness against cold during development of diverse poikilothermic organisms, probably by preventing the accumulation of the ESCRT-III core component Shrub on the endosomal membrane.