RADIOMETRIC SELECTIVE-INHIBITION TESTS FOR DIFFERENTIATION OF MYCOBACTERIUM-TUBERCULOSIS, MYCOBACTERIUM-BOVIS, AND OTHER MYCOBACTERIA
RADIOMETRIC SELECTIVE-INHIBITION TESTS FOR DIFFERENTIATION OF MYCOBACTERIUM-TUBERCULOSIS, MYCOBACTERIUM-BOVIS, AND OTHER MYCOBACTERIA
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DOI：
10.1128/jcm.21.4.565-568.1985
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发表时间：
1985-01-01
影响因子：
9.4
通讯作者：
HAWKINS, JE
中科院分区：
文献类型：
--
作者：
GROSS, WM;HAWKINS, JE
在繁忙的参考实验室中，评估放射性选择性抑制试验以快速区分结核分枝杆菌和博伊斯分枝杆菌以及结核分枝杆菌复合体与其他分枝杆菌，使用5μg的对硝基-α-乙酰氨基-β-羟基苯丙酮和62.5和125μg/ml的7H12培养基的盐酸羟胺来分离结核分枝杆菌复合体。来自其他分枝杆菌（MOTT 杆菌）的结核菌复合体。由于在流行病学上区分结核分枝杆菌和牛分枝杆菌很重要，因此还通过放射测定法测定了对1μg噻吩-2-羧酸/ml的敏感性。通过使用这 3 种试剂作为选择性抑制剂，通过 BACTEC 放射测定程序高度特异性地区分结核分枝杆菌、牛分枝杆菌和 MOTT 杆菌。对提交给该参考实验室的固体培养基上的临床分离株进行的测试结果可在 5 天内获得。
In the context of a busy reference laboratory, radiometric selective inhibition tests were evaluated for rapid differentiation of M. tuberculosis and M. boyis and of the M. tuberculosis complex from other mycobacteria, p-Nitro-.alpha.-acetylamino-.beta.-hydroxypropiophenone at 5 .mu.g and hydroxylamine hydrochloride at 62.5 and 125 .mu.g/ml of 7H12 medium were used to separate the M. tuberculosis complex from other mycobacteria (MOTT bacilli). Since it is important epidemiologically to distinguish M tuberculosis from M. bovis, susceptibility to 1 .mu.g of thiophene-2-carboxylic acid/ml was also determined radiometrically. By using these 3 agents as selective inhibitors, M. tuberculosis, M. bovis and MOTT bacilli were differentiated with a high degree of specificity by a BACTEC radiometric procedure. Results of tests performed on clinical isolates submitted on solid medium to this reference laboratory were avialable within 5 days.