Isolation and Sequence Analysis of the Sox-1, -2, -3 Homologs in Trionyx sinensis and Alligator sinensis Having Temperature-Dependent Sex Determination
Isolation and Sequence Analysis of the Sox-1, -2, -3 Homologs in Trionyx sinensis and Alligator sinensis Having Temperature-Dependent Sex Determination
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DOI：
10.1007/s10528-006-9019-0
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发表时间：
2006-05
影响因子：
2.4
通讯作者：
Jifang Zheng;Mu-yuan Zhu
中科院分区：
文献类型：
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作者：
Jifang Zheng;Mu-yuan Zhu
Sox基因家族成员的特征是HMG-box，它与小鼠睾丸决定基因Sry的序列相似。利用简并引物聚合酶链式反应，从中华鳖(TS41-47)和扬子鳄(AS41-48)基因组DNA中克隆了7个和8个Sry相关基因的HMG-box基序，并进行了TSD性别测定。BLAST分析表明，在15个Sry相关基因中，TS41、TS42、AS41和AS42与小鼠Sox-1、-2和-3基因的HMG-box结构域的氨基酸同源性分别为80%、72%、81%和79%。分子系统发育分析表明，TS41-42和AS41-42与非爬行动物脊椎动物Sox-1、-2、-3同源物的聚类距离较远，包括鱼类、两栖动物、鸟类和哺乳动物。TS41-42、AS41-42和非爬行动物脊椎动物SOX-1、-2、-3同源物的氨基酸同源性低于SOX-1、-2、-3同源物，提示中华鳖TS41-42和扬子鳄AS41-42的序列变化可能发生在羊膜生物多样性之后。
Members of the Sox gene family are characterized by an HMG-box that shows sequence similarity with that of the mouse testis-determining gene Sry. Using degenerate primers PCR, seven and eight HMG-box motifs of Sry-related genes were cloned and sequenced from genomic DNA of Trionyx sinensis (termed TS41-47) and Alligator sinensis (AS41-48) with TSD (temperature-dependent sex determination). Among 15 Sry-related genes, TS41, TS42, AS41, and AS42 shared 80, 72, 81, and 79% amino acid identity, respectively, with each HMG-box domain of the mouse Sox-1, -2, and -3 genes by Blast analysis. Molecular phylogenetic analysis showed that the clustering of TS41-42 and AS41-42 was distant to the clustering of the nonreptilian vertebrate Sox-1, -2, -3 homologs, including fish, amphibian, bird, and mammals. The amino acid identity among TS41-42, AS41-42, and the nonreptilian vertebrate Sox-1, -2, -3 homologs is lower than identities among the Sox-1, -2, -3 homologs, suggesting that the sequence changes in TS41-42 of Trionyx sinensis and AS41-42 of Alligator sinensis might have occurred after the diversification of amniotes.