Expression of Aedes trypsin-modulating oostatic factor on the virion of TMV:: A potential larvicide
Expression of Aedes trypsin-modulating oostatic factor on the virion of TMV:: A potential larvicide
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DOI：
10.1073/pnas.0606146103
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发表时间：
2006-12-12
影响因子：
11.1
通讯作者：
DeLoof, Arnold
中科院分区：
文献类型：
--
作者：
Borovsky, Dov;Rabindran, Shailaja;DeLoof, Arnold
我们报告了烟草花叶病毒（TMV）病毒粒子表面的工程与蚊子去顶膜激素，胰蛋白酶调节促卵生长因子（TMOF）。TMV外壳蛋白（CP）通过使用通读、泄漏终止密码子在C末端融合到TMOF，所述终止密码子促进CP和嵌合CP-TMOF（20：1比率）的表达，所述CP和嵌合CP-TMOF在感染的烟草中共组装成病毒颗粒。用杂交TMV RNA感染的植物积累TMOF至总可溶性蛋白的1.3%的水平。感染烟草叶盘，喂养烟夜蛾四龄幼虫，阻碍他们的生长，并抑制胰蛋白酶和糜蛋白酶的活性在他们的中肠。纯化的CP-TMOF病毒颗粒喂养蚊子幼虫停止幼虫生长并导致死亡。由于TMV具有广泛的宿主范围，在植物中表达TMV-TMOF可用作保护植物免受农业害虫和控制媒介蚊子的一般方法。
We report the engineering of the surface of the tobacco mosaic virus (TMV) virion with a mosquito decapepticle hormone, trypsinmodulating oostatic factor (TMOF). The TMV coat protein (CP) was fused to TMOF at the C terminus by using a read-through, leaky stop codon that facilitated expression of CP and chimeric CP-TMOF (20:1 ratio) that were coassembled into virus particles in infected Nicotiana tabacum. Plants that were infected with the hybrid TMV RNA accumulated TMOF to levels of 1.3% of total soluble protein. Infected tobacco leaf discs that were fed to Heliothis virescens fourth-instar larvae stunted their growth and inhibited trypsin and chymotrypsin activity in their midgut. Purified CP-TMOF virions fed to mosquito larvae stopped larval growth and caused death. Because TMV has a wide host range, expressing TMV-TMOF in plants can be used as a general method to protect them against agricultural insect pests and to control vector mosquitoes.