Genome comparison of a novel foot-and-mouth disease virus with other FMDV strains
Genome comparison of a novel foot-and-mouth disease virus with other FMDV strains
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DOI：
10.1016/j.bbrc.2004.08.086
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发表时间：
2004-10-08
影响因子：
3.1
通讯作者：
Chen, JG
中科院分区：
文献类型：
--
作者：
Feng, Q;Yu, H;Chen, JG
新的口蹄疫病毒HKN/2002的基因组全长为8104个核苷酸(M)(不包括聚(C)链和聚(A)尾)，由1042个核苷酸的5‘非翻译区、6966个核苷酸的开放阅读框和93个核苷酸的3’非翻译区组成。比较了HKN/2002和其他已知的口蹄疫病毒株的基因组序列。基于VP1、VP2和VP3的相邻连接(NJ)树根据不同的血清型被划分为不同的聚类群，而其他基于区域的NJ树在不同的血清型之间表现出一定程度的交叉。在HKN/2002中发现了突变，包括VP1 G-H环的频繁缺失和插入，以及3A蛋白中10个氨基酸残基的缺失。推测了HKN/2002与1970年从香港猪宿主分离的亚洲口蹄疫病毒谱系的进化关系。在HKN/2002的5‘非编码区中发现的43个核苷酸缺失可能是导致一个伪结构域丢失的原因。(C)2004 Elsevier Inc.保留所有权利。
The genome of a novel foot-and-mouth disease virus, HKN/2002, was 8104 nucleotides (m) in length (excluding the poly(C) tract and poly(A) tail) and was composed of a 1042-nt 5'-untranslated region (UTR), a 6966-nt open reading frame, and a 93-nt 3'-UTR. Genome sequences of HKN/2002 and other known FMDV strains were compared. The VP1, VP2, and VP3-based neighbor-joining (NJ) trees were divided into distinct clusters according to different serotypes, while other region-based NJ trees exhibited some degree of intercross among serotypes. Mutations in HKN/2002 were revealed, including frequent deletions and insertions in the G-H loop of VP1, and deletion involving 10 amino acid residues in the 3A protein. An evolutionary relationship of HKN/2002 with an Asian FMDV lineage isolated from a Hong Kong swine host in 1970 was postulated. A 43-nt deletion identified in the 5'-UTR of HKN/2002 possibly contributed to the loss of one pseudo-knot domain. (C) 2004 Elsevier Inc. All rights reserved.