Systematic Localization Study on Novel Proteins Encoded by Meiotically Up-Regulated ORFs in Fission Yeast
Systematic Localization Study on Novel Proteins Encoded by Meiotically Up-Regulated ORFs in Fission Yeast
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DOI：
10.1271/bbb.110558
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发表时间：
2011-12-01
影响因子：
1.6
通讯作者：
Toda, Takashi
中科院分区：
文献类型：
--
作者：
Ikebe, Chiho;Konishi, Manabu;Toda, Takashi
我们进行了有丝分裂定位研究的基因产物编码的56个未知的裂殖酵母ORF的转录上调在减数分裂分裂。尽管减数分裂基因诱导，这些基因在有丝分裂过程中表达。七个基因产物定位在细胞核和/或染色质中;另一个是有丝分裂特异性纺锤体极体成分，有趣的是，它的人类同源物也定位在培养的HeLa细胞的中心体中。两个产品似乎是本地化的细胞质微管，而四个线粒体蛋白。其他三种蛋白质被发现在内侧环胞质分裂后，另一个是定位在整个细胞周边。其余38个蛋白质在细胞质中检测到，并表现出不同的空间格局。这一系统的研究有助于我们全面了解裂殖酵母作为真核生物模型的所有蛋白质功能。
We conducted a mitotic localization study on gene products encoded by 56 uncharacterized fission yeast ORFs that were transcriptionally up-regulated during meiotic division. Despite meiotic gene induction, these genes were expressed during mitosis as well. Seven gene products were localized in the nucleus and/or chromatin; another one was a mitosis-specific spindle pole body component and, intriguingly, its human homologue was also localized in the centrosome of cultured HeLa cells. Two products appeared to be localized in cytoplasmic microtubules, whereas four were mitochondrial proteins. Three other proteins were found in the medial ring upon cytokinesis and another was localized on the entire cell periphery. The remaining 38 proteins were detected in the cytoplasm and showed varied spatial patterns. This systematic study helps our integrated understanding of all the protein functions in the fission yeast as a eukaryotic model.