Convectively driven polymerase chain reaction thermal cycler
Convectively driven polymerase chain reaction thermal cycler
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DOI：
10.1021/ac034941g
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发表时间：
2004-07-15
影响因子：
7.4
通讯作者：
Milanovich, F
中科院分区：
文献类型：
--
作者：
Wheeler, EK;Benett, W;Milanovich, F
我们已经制造了一个低成本的一次性聚合酶链反应热室，使用浮力移动的样品溶液之间的扩增所需的不同温度。三维，非稳态有限元建模和一个简单的1-D稳态模型一起使用的数字粒子图像测速数据来表征设备内的流动。生物样品已经使用这种新型的热室扩增。放大时间不到30分钟。更重要的是，能耗分析显示，与当前技术相比，有了显着改进。
We have fabricated a low-cost disposable polymerase chain reaction thermal chamber that uses buoyancy forces to move the sample solution between the different temperatures necessary for amplification. Three-dimensional, unsteady finite element modeling and a simpler 1-D steady-state model are used together with digital particle image velocimetry data to characterize the flow within the device. Biological samples have been amplified using this novel thermal chamber. Time for amplification is less than 30 min. More importantly, an analysis of the energy consumption shows significant improvements over current technology.