Genetic immunization of rainbow trout (Oncorhynchus mykiss) against infectious hematopoietic necrosis virus.
Genetic immunization of rainbow trout (Oncorhynchus mykiss) against infectious hematopoietic necrosis virus.
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发表时间：
1996-06
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通讯作者：
E. Anderson;D. Mourich;S. Fahrenkrug;S. Lapatra;J. Shepherd;J. Leong
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作者：
E. Anderson;D. Mourich;S. Fahrenkrug;S. Lapatra;J. Shepherd;J. Leong
用巨细胞病毒启动子控制的编码传染性造血细胞坏死病毒（IHNV）核蛋白或糖蛋白基因的质粒载体免疫虹鳟鱼（Oncorhynchus mykiss）对抗IHNV。将质粒DNA注射到虹鳟鱼苗的骨骼肌中，通过检测病毒中和抗体和酶联免疫吸附试验抗体活性，以及对活病毒攻击的保护力来确定免疫效果。单独或与核蛋白编码质粒pCMV 4-N组合注射糖蛋白编码质粒pCMV 4-G的鱼产生糖蛋白特异性和病毒中和抗体应答。接种疫苗的鱼也被保护免受随后的IHNV攻击。单独接受pCMV 4-N的鱼不产生可测量的病毒特异性抗体，并且被IHNV感染杀死。这些研究表明，DNA疫苗接种将保护虹鳟鱼免受IHNV感染的致命影响。
Plasmid vectors encoding the infectious hematopoietic necrosis virus (IHNV) nucleoprotein or glycoprotein gene under the control of a cytomegalovirus promoter were used to immunize rainbow trout (Oncorhynchus mykiss) against IHNV. The plasmid DNA was injected into the skeletal muscle of rainbow trout fry, and immunization was determined by the detection of virus-neutralizing and enzyme-linked immunosorbent assay antibody activity, and by protection against live virus challenge. Fish injected with the glycoprotein-encoding plasmid pCMV4-G, either alone or in combination with the nucleoprotein-encoding plasmid pCMV4-N, generated glycoprotein-specific and virus-neutralizing antibody responses. The vaccinated fish were also protected from subsequent IHNV challenge. Fish receiving pCMV4-N alone did not produce measurable virus-specific antibody and were killed by IHNV infection. These studies show that DNA vaccination will protect rainbow trout against the lethal effects of IHNV infection.