Eosinophil chemotactic lymphokine produced by spleen cells of Schistosoma japonicum-infected mice. III. Isolation and characterization of two distinctive eosinophil chemotactic lymphokines directed against different maturation stages of eosinophils.
Eosinophil chemotactic lymphokine produced by spleen cells of Schistosoma japonicum-infected mice. III. Isolation and characterization of two distinctive eosinophil chemotactic lymphokines directed against different maturation stages of eosinophils.
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发表时间：
1987
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通讯作者：
M. Owhashi;Y. Nawa
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作者：
M. Owhashi;Y. Nawa
用DE 52阴离子交换层析、Procion Red琼脂糖亲和层析和高效液相色谱从日本血吸虫感染小鼠脾细胞条件培养液中分离出两种特异性的嗜酸性粒细胞趋化性淋巴因子（ECF-L），分别针对骨髓来源的嗜酸性粒细胞（BM-Eo）和腹腔来源的嗜酸性粒细胞（PEC-Eo）。经SDS-PAGE分析，ECF-L对BM-Eo的相对分子质量为10，000，对PEC-Eo的相对分子质量为46，000。分离后，使用BM-Eo、PEC-Eo和循环Eo检查两种不同ECF-L的选择性。针对BM-Eo的ECF-L仅能吸引BM-Eo，而针对PEC-Eo的ECF-L不仅能吸引PEC-Eo，还能吸引循环Eo。合成的ECF-A对BM-和循环-Eo具有趋化性，但对PEC-Eo没有趋化性。当BM-Eo与对PEC-Eo具有选择性的ECF-L一起培养时，它们变得与对PEC-Eo具有选择性的ECF-L反应，而对BM-Eo具有选择性的ECF-L没有这种效果。这些结果表明ECF-L对PEC-Eo的选择性不仅是一种化学引诱物，而且在嗜酸性粒细胞分化中也很重要。
Two distinctive types of eosinophil chemotactic lymphokines (ECF-L) directed against eosinophils obtained from bone marrow (BM-Eo) or from peritoneal cavity (PEC-Eo) were isolated from the media conditioned by spleen cells from mice infected with Schistosoma japonicum by a combination of anion-exchange chromatography on DE52, affinity chromatography on Procion Red agarose, and high-performance liquid chromatography. The molecular weight of ECF-L directed against BM-Eo was 10,000 and that against PEC-Eo was 46,000 by SDS-PAGE analysis. After isolation, selectivity of the two distinct ECF-L was examined using BM-Eo, PEC-Eo and circulating Eo. ECF-L directed against BM-Eo could attract only BM-Eo, whereas that directed against PEC-Eo could attract not only PEC-Eo but also circulating Eo. Synthetic ECF-A was chemotactic against both BM- and circulating-Eo but not against PEC-Eo. When BM-Eo were cultured with ECF-L selective to PEC-Eo, they became reactive to ECF-L selective to PEC-Eo, whereas ECF-L selective to BM-Eo did not have such effect. These results suggest that ECF-L selective to PEC-Eo is not simply a chemoattractant but also is important in eosinophil differentiation.