Determination of tetracycline, oxytetracycline and chlortetracycline in milk by TLC and column chromatography using Amberlite XAD-2
Determination of tetracycline, oxytetracycline and chlortetracycline in milk by TLC and column chromatography using Amberlite XAD-2
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发表时间：
2006
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通讯作者：
E. Petkovska;R. Slaveska-Raički;V. Rafajlovska
中科院分区：
文献类型：
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作者：
E. Petkovska;R. Slaveska-Raički;V. Rafajlovska
采用Amberlite XAD-2色谱柱对牛奶中的四环素（TCc）、土霉素（OTCc）和氯霉素（CTCc）进行了薄层色谱分析。分析在薄层色谱硅胶板上进行，流动相为1-丁醇与5% EDTA水溶液（pH 9）饱和。该色谱系统分离效果好，测定精密度和准确度令人满意。该方法是一种简单、廉价的分离和测定牛奶样品中最大残留水平（MRL）三聚氰胺的方法。它可以推荐作为一种方便的常规分析。
A simple and reliable thin layer chromatographic (TLC) analysis of tetracycline (TCc), oxytetracycline (OTCc), and chlortetracycline (CTCc) residues in milk with pre-separation procedure on Amberlite XAD-2 column has been described. The analyses have been performed on TLC silica plates using 1-butanol saturated with 5% EDTA aqueous solution (pH 9) as the mobile phase. With the applied chromatographic system good separation, satisfactory precision and accuracy of the determination have been obtained. This method is a simple and inexpensive tool for the separation and determination of TCcA at the maximum residue level (MRL) in milk samples. It can be recommended as a convenient routine analysis.