Focusing through dynamic tissue with millisecond digital optical phase conjugation.
Focusing through dynamic tissue with millisecond digital optical phase conjugation.
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DOI：
10.1364/optica.2.000728
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发表时间：
2015-08-20
期刊：
影响因子：
10.4
通讯作者：
Yang C
中科院分区：
文献类型：
--
作者：
Wang D;Zhou EH;Brake J;Ruan H;Jang M;Yang C
数字光学相位共轭（DOPC）是一种用于波前整形和相位共轭的新技术，用于将光聚焦通过生物组织等散射介质。DOPC特别有吸引力，因为与非线性光学方法相比，它本质上实现了高通量反射率。然而，液晶空间光调制器的慢刷新速率和计算机数据传输速度所施加的限制迄今为止使得DOPC难以实现短于~200 ms的回放延迟，并且因此阻止DOPC实际应用于厚的活体样本。在本文中，我们报告了一种新的DOPC系统，能够为5.3毫秒的播放延迟。这种速度提高了近2个数量级，是通过使用一个数字的FPGA器件，现场可编程门阵列（FPGA）处理，和一个单发二进制相位恢复技术。有了这个系统，我们能够通过2.3毫米的活体小鼠皮肤与血液流动通过它（去相关时间~30毫秒），并证明了重点可以保持不确定的一个重要的技术里程碑，以前没有报道，以我们所知。
Digital optical phase conjugation (DOPC) is a new technique employed in wavefront shaping and phase conjugation for focusing light through or within scattering media such as biological tissues. DOPC is particularly attractive as it intrinsically achieves a high fluence reflectivity in comparison to nonlinear optical approaches. However, the slow refresh rate of liquid crystal spatial light modulators and limitations imposed by computer data transfer speeds have thus far made it difficult for DOPC to achieve a playback latency of shorter than ~200 ms and, therefore, prevented DOPC from being practically applied to thick living samples. In this paper, we report a novel DOPC system that is capable of 5.3 ms playback latency. This speed improvement of almost 2 orders of magnitude is achieved by using a digital micromirror device, field programmable gate array (FPGA) processing, and a single-shot binary phase retrieval technique. With this system, we are able to focus through 2.3 mm living mouse skin with blood flowing through it (decorrelation time ~30 ms) and demonstrate that the focus can be maintained indefinitely—an important technological milestone that has not been previously reported, to the best of our knowledge.