Purification and partial characterization of a new antitumor protein from Tegillarca granosa.
Purification and partial characterization of a new antitumor protein from Tegillarca granosa.
复制标题
粒泥新抗肿瘤蛋白的纯化和部分表征
DOI：
10.3390/md13031466
复制
发表时间：
2015-03-17
期刊：
影响因子：
5.4
通讯作者：
Yu R
中科院分区：
文献类型：
--
作者：
Lv S;Gao J;Liu T;Zhu J;Xu J;Song L;Liang J;Yu R
从海洋生物泥蚶Tegillarca granosa L.通过阴离子交换和疏水色谱法。经RP-HPLC测定，D2-3的纯度大于99.0%。ESI-MS/MS测得其分子量为20.320 kDa，D2-3的等电点为4.70。采用MTT法测定D2-3对4种人肿瘤细胞株的抗肿瘤活性。通过紫外-可见光谱、红外光谱和圆二色谱对D2-3的构象进行了表征。经MALDI-TOF-MS和de novo序列测定，D2-3的部分氨基酸序列为LMMTDVEESR、SSHMLSECRRK、KNGRNVDISHKDKG、SSDPTLMDPDDTNKDR、SSDKNTCSKTEYYTR和SSETMPYDVLDTNEMR。
A new protein, coded as D2-3, was obtained from the marine organism Tegillarca granosa L. by anion exchange and hydrophobic chromatography. The purity of D2-3 was over 99.0% as measured by RP-HPLC. Its molecular weight was shown to be 20.320 kDa by ESI-MS/MS, and the isoelectric point of D2-3 was 4.70. The antitumor activity of D2-3 against four human tumor cell lines was measured by MTT assay. The conformational structure of D2-3 was further characterized by UV-vis, FT-IR and CD spectroscopy. Partial amino acid sequences of D2-3 were determined to be LMMTDVEESR, SSHMLSECRRK, KNGRNVDISHKDKG, SSDPTLMDPDDTNKDR, SSDKNTCSKTEYYTR and SSETMPYDVLDTNEMR via MALDI-TOF-MS and de novo sequencing.